风湿性二尖瓣狭窄伴心房颤动患者血红素氧合酶-1的表达

任贝贝; 郭俊杰; 王雪春; 李永红

doi:10.13201/j.issn.1001-1439.2013.03.014

风湿性二尖瓣狭窄伴心房颤动患者血红素氧合酶-1的表达

- 1. 青岛大学医学院(山东青岛, 266003);
- 2. 青岛大学医学院附属医院心内科
基金项目:
青岛市科学技术局资助项目(No:02-2-kj-yn-25)

详细信息

通讯作者: 李永红,E-mail:liyonghong-66@163.com

中图分类号: R541.7

收稿日期: 2012-10-22

Expression of heme oxygenates-1 in atrial fibrillation patients with rheumatic mitral stenosis

- 1. Medical College, Qingdao University, Qingdao, Shandong, 266003, China;
- 2. Department of Cardiology, the Affiliated Hospital of Medical College, Qingdao University

More Information

Corresponding author: LI Yonghong, E-mail: liyonghong-66@163.com

Received Date: 22 October 2012

摘要

摘要: 目的:观察血红素氧合酶(HO)-1与风湿性二尖瓣狭窄伴心房颤动(房颤)患者心肌重构的关系,探讨HO-1在房颤心肌重构中的作用及机制。方法:选取风湿性心瓣膜病行换瓣手术患者60例,将其分为窦性心律组(21例)及房颤组(39例),术前常规行心电图、超声心动图、X线等相关检查。外科换瓣手术时取右心耳组织约200mg,通过组织学检查、逆转录聚合酶链反应和Western blot技术分别检测心肌纤维化程度、HO-1及丝裂原活化蛋白激酶p38的表达水平。结果:①HE染色及Masson染色显示,房颤组心肌纤维化程度较窦性心律组显著;②与窦性心律组相比,房颤组HO-1mRNA及蛋白表达均明显上调,p38蛋白表达明显升高(均P<0.05)。结论:风湿性心脏病房颤患者心房重构过程中,HO-1和p38的表达均显著升高;HO-1表达增高可能与p38通路的激活有关。
- 心房颤动 /
- 血红素氧合酶-1 /
- 丝裂原活化蛋白激酶p38
Abstract: Objective:To study the relationship between HO-1 and fibrosis of atrial fibrillation(Af) in rheumatic mitral stenosis patients,and investigate the effect of HO-1 in fibrosis of Af.Method:Patients with rheumatic heart disease who needed valve replacements were divided into sinus rhythm group(n=21) and Af group(n=39).Routine examinations,such as electrocardiogram,echocardiogram and X-ray were taken before the operations.During the operation,200 mg right atrial tissue was cut down to test the expression of HO-1,the level of p38 and the process of intimal fibrosis by RT-PCR technique,Western blot,HE stain and Masson stain,respectively.Result:①HE stain and Masson stain showed more myocardial fibrosis in Af group.②Compared with sinus rhythm group,the mRNA expression of HO-1 and p38 were obviously upregulated in Af group(P<0.05).Conclusion:The expression of HO-1 and p38 are both elevated in Af patients with rheumatic heart disease.The effect of HO-1 in Af may be related to the activation of p38 passageway.
- atrial fibrillation /
- heme oxygenates-1 /
- mitogen-activated protein kinases p38

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