Research progress in differentiation strategy of induced pluripotent stem cells into cardiomyocytes
-
摘要: 诱导多能干细胞(iPSCs)是由多种体细胞重编程而获得的,具有与胚胎干细胞(ESCs)相似的特性,能自我更新、无限增殖和多向分化的特性。但目前iPSCs向心肌细胞自发分化的效率非常低。如何才能有效地诱导iPSCs向心肌细胞的定向分化,对深入认识心肌细胞发生和发育的机制及实现其在药物筛选和心血管疾病治疗中的作用均具有非常重要的意义。本文重点对近年来iPSCs向心肌细胞分化策略的研究进展进行综述。Abstract: Similar to embryonic stem cells(ESCs),induced pluripotent stem cells(iPSCs),obtained by somatic cell reprogramming,are capable of self-renewal,unlimited growth and multi-direction differentiation.Obviously,spontaneous differentiation of iPSCs into cardiomyocytes is limited.To illuminate the mechanisms of and effectively differentiate iPSCs into cardiomyocytes are crucial to obtain fundamental knowledge in screening drugs and promoting the usage in cardiovascular disease therapy.This article reviewed the recent research about the strategies of differentiation potential of iPSCs into cardiomyocytes in vitro models.
-
Key words:
- induced pluripotent stem cells /
- cardiomyocytes /
- differentiation
-
-
[1] IEDA M,FU J D,DELGADO-OLGUIN P,et al.Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors[J].Cell,2010,142:375-386.
[2] EFE J A,HILCOVE S,KIM J,et al.Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy[J].Nat Cell Biol,2011,13:215-222.
[3] SCHENKE-LAYLAND K,RHODES K E,ANGE-LIS E,et al.Reprogrammed mouse fibroblasts differentiate into cells of the cardiovascular and hematopoietic lineages[J].Stem Cells,2008,26:1537-1546.
[4] ZHANG J H,WILSON G F,SOERENS A G,et al.Functional cardiomyocytes derived from human induced pluripotent stem cells[J].Circ Res,2009,104:e30-e41.
[5] NARAZAKI G,UOSAKI H,TERANISHI M,et al.Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells[J].Circulation,2008,118:498-506.
[6] SHAV D,EINAV S.The effect of mechanical loads in the differentiation of precursor cells into mature cells[J].Ann N Y Acad Sci,2010,1188:25-31.
[7] KURPINSKI K,PARK J,THAKAR R G,et al.Regulation of vascular smooth muscle cells and mesenchymal stem cells by mechanical strain[J].Mol Cell Biomech,2006,3:21-34.
[8] SHAFA M,KRAWETZ R,ZHANG Y,et al.Impact of stirred suspension bioreactor culture on the differentiation of murine embryonic stem cells into cardiomyocytes[J].BMC Cell Biol,2011,12:53.doi:10.1186/1471-2121-12-53.
[9] SHIMIZU N,YAMAMOTO K,OBI S,et al.Cyclic strain induces mouse embryonic stem cell differentiation into vascular smooth muscle cells by activating PDGF receptor beta[J].J Appl Physiol,2008,104:766-772.
[10] ADAMO L,NAVEIRAS O,WENZEL P L,et al.Biomechanical forces promote embryonic haematopoiesis[J].Nature,2009,459:1131-1135.
[11] 薛媛媛,龚惠,闫媛,等.机械牵张对诱导多能干细胞向心肌细胞分化的研究[J].中国分子心脏病学杂志,2009,12(6):350-355.
[12] PAQUIN J,DANALACHE B A,JANKOWSKI M,et al.Oxytocin induces differentiation of P19 embryonic stem cells to cardiomyocytes[J].PNAS,2002,99:9550-9555.
[13] 王洋,沈慧娟,周敏芬,等.心肌条件培养液与5-氮胞苷诱导骨髓基质干细胞向心肌样细胞的分化[J].中国组织工程研究与临床康复,2011,15(23):4203-4206.
[14] 张卫泽,李国庆,陈永清,等.5-氮胞苷诱导脂肪间充质干细胞向心肌细胞分化的毒理遗传学研究[J].第四军医大学学报,2009,30(23):2820-2823.
[15] 陈冬梅,吕长荣,辛晓玲,等.牛诱导性多能干细胞向心肌细胞的诱导分化[J].西北农林科技大学学报,2010,38(10):1-6.
[16] QUATTROCELLI M,PALAZZOLO G,AGNOLINI,et al.Synthetic sulfonyl-hydrazone-1 positively regulates cardiomyogenic microRNA expression and cardiomyocyte differentiation of induced pluripotent stem cells[J].Cell Biochem,2011,112:2006-2014.
[17] FUJIWARA M,YAN P,OTSUJI T G,et al.Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-A[J].PLoS One,2011,2:e16734.doi:10.1371/journal.pone.0016734.
[18] CAO N,LIU Z,CHEN Z,et al.Ascorbic acid enhances the cardiac differentiation of induced pluripotent stem cells through promoting the proliferation of cardiac progenitor cells[J].Cell Res,2012,22:219-236.
[19] LIM S Y,SIVAKUMARAN P,CROMBIE D E,et al.Trichostatin A Enhances Differentiation of Human Induced Pluripotent Stem Cells to Cardiogenic Cells for Cardiac Tissue Engineering[J].Stem Cells Translational Medicine,2013,2:715-725.
[20] 王秀丽,王常勇,虞星炬,等.二甲基亚讽诱导胚胎干细胞分化伴随凋亡发生[J].解剖学杂志,2005,28(6):609-614.
[21] PISANO F,ALTOMARE C,CERVIO E,et al.Combination of miRNA499 and miRNA133 exerts a synergi c effect on cardiac differentiation[J].Stem Cells,2015,33:1187-1199.
[22] CALLIS T E,PANDYA K,SEOK H Y,et al.MicroRNA-208ais a regulator of cardiac hypertrophy and conduction in mice[J].The Journal of Clinical Investigation,2009,119:2772-2786.
[23] 吴仪,陈虎艳,李宁,等.microRNA定向诱导iPS细胞向心肌细胞分化的初步研究[J].宁夏医科大学学报,2012,8(34):760-763.
[24] 丁璐,李晓莉,曾迪,等.microRNA-1和microRNA-133参与小鼠用到多能干细胞向心肌的分化[J].心脏杂志,2015,27(6):634-644.
[25] 戴波,李洪涛,刘泽,等.钠钙交换体启动子促进诱导多能干细胞向心肌细胞转化[J].中国组织工程研究,2014,18(19):3069-3074.
[26] CHAN S S,LI H J,HSUEH Y C,et al.Fibroblast growth factor-10promotes cardiomyocyte differentiation from embryonic and induced pluripotent stem cells[J].PLoS One,2010,5:e14414.doi:10.1371/journal.pone.0014414.
[27] KATTMAN S J,WITTY A D,GAGLIARDI M,et al.Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines[J].Cell Stem Cell,2011,8:228-240.
[28] REN Y M,LEE M Y,SCHLIFFKE S,et al.Small molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells[J].J Mol Cell Cardiol,2011,51:280-287.
[29] 赵霞,张晓刚,熊挺淋,等.血管内皮生长因子对诱导多能干细胞向心肌细胞分化的影响[J].中国老年学杂志,2012,32(4):755-758.
[30] 马珂,刘通,黄炜,等.Apelin促进人诱导性多能干细胞心肌定向分化的研究[J].中华老年心脑血管病杂志,2014,16(1):73-77.
[31] 刘通,卜庆婷,马珂,等.心肌营养素-1诱导小鼠诱导性多能干细胞心肌细胞定向分化的实验研究[J].心脏杂志,2013,25(3):307-322.
[32] WERNIG M,MEISSNER A,CASSADY J P,et al.c-Myc is dispensable for direct re programming of mouse fibroblasts[J].Cell Stem Cell,2008,2:10-12.
[33] MARTINEZ-FERNANDEZ A,NELSON T J,IKE-DA Y,et al.c-MYC-Independent Nuclear Reprogramming Favors Cardiogenic Potential of Induced Pluripotent Stem Cells[J].J Cardiovasc Translat Res,2010,3:13-23.
[34] CHIN M H,MASON M J,XIE W,et al.Induced pluripotent stem cells and embryonic stem cells are distinguished bygene expression signatures[J].Cell Stem Cell,2009,5:111-123.
-
计量
- 文章访问数: 419
- PDF下载数: 49
- 施引文献: 0
下载: