内皮型一氧化氮合成酶基因rs3918181多态性与贵州省苗族、布依族原发性高血压的关系

张旺德; 何燕; 赵孝梅; 张婷; 王婵娟; 禹文峰; 官志忠

doi:10.13201/j.issn.1001-1439.2017.05.012

内皮型一氧化氮合成酶基因rs3918181多态性与贵州省苗族、布依族原发性高血压的关系

- 地方病与少数民族疾病教育部重点实验室(贵州医科大学) 贵州省医学分子生物学重点实验室(贵州医科大学)(贵阳, 550004)
基金项目:
国家科技支撑计划项目（No：2013BAI05B03）；贵州省“2011协同创新中心”[No：黔教合协同创新中心（2014）06号]

详细信息

通讯作者: 何燕,E-mail:annieheyan@gmc.edu.cn

中图分类号: R544.1

收稿日期: 2016-10-09

修回日期: 2016-12-16

The relationship between rs3918181 polymorphism of endothelial nitric oxide synthase gene and essential hypertension in Miao and Buyi nationality of Guizhou

- Key Laboratory of Endemic and Ethnic Disease (Guizhou Medical University), Ministry of Education;Key Laboratory of Medical Molecular Biology (Guizhou Medical University), Guiyang, 550004, China

More Information

Corresponding author: HE Yan, E-mail: annieheyan@gmc.edu.cn

Received Date: 09 October 2016

Revised Date: 16 December 2016

摘要

摘要: 目的：探讨贵州省苗族、布依族人群内皮型一氧化氮合成酶（eNOS）基因第14内含子rs3918181位点多态性与原发性高血压的关系。方法：选取贵州雷山县苗族和荔波县布依族247例原发性高血压患者（苗族123例，布依族124例）为高血压组，244例健康人群（苗族118例，布依族126例）为对照组。采用TaqMan探针法检测eNOS基因rs3918181位点多态性，分析rs3918181位点不同基因型及等位基因频率在两个民族人群中的分布。结果：eNOS基因rs3918181位点AA、AG、GG基因型及A、G等位基因在苗族、布依族高血压组与对照组中的分布频率符合Hardy-Weinberg平衡定律；贵州苗族、布依族人群eNOS基因rs3918181位点基因型分布频率及等位基因频率在高血压组和健康对照组之间差异无统计学意义。结论：eNOS基因rs3918181位点多态性与贵州省苗族、布依族人群原发性高血压可能不相关，该位点多态性在贵州苗族、布依族人群之间无民族差异性。
- 原发性高血压 /
- 一氧化氮合酶 /
- 基因多态性 /
- 贵州
Abstract: Objective: To investigate the relationship between polymorphism of endothelial nitric oxide synthase (eNOS) gene intron 14 th rs3918181 locus and essential hypertension (EH) in Miao and Buyi nationalities in Guizhou Province. Method: A total of 247 cases with hypertension (123 cases of Miao and 124 cases of Buyi) was enrolled in the study, and 244 cases of healthy people (118 cases of Miao and 126 cases of Buyi) served as controls, above all of subjects were came from Leishan and Libo county in Guizhou. TaqMan probe technique was used for the detection of eNOS rs3918181 SNP, and the distribution of different genotype and allele frequency in the two nationality were analyzed. Result: The frequency distribution in eNOS,which each genotype and allele in EH and control groups of the Miao and Buyi nationalities, were in accorded with the Hardy-Weinberg genetic equilibrium law. There was no significant difference in frequency of genotypes and allele between EH and control groups.Conclusion: The rs3918181 loci gene polymorphism of eNOS gene is may not be associated with EH susceptibility in Miao and Buyi population of Guizhou Province, and no racial difference in polymorphism between Miao and Buyi nationality in Guizhou.
- hypertension /
- nitric oxide synthase /
- genetic polymorphism /
- Guizhou

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参考文献(22)

[1]	TANIRA M O, A L BALUSHI K A. Genetic variations related to hypertension:a review[J]. J Hum Hypertens, 2005, 19:7-19.
[2]	LI K, LIANG Y, SUN Y, et al. The relationship between polymorphisms at 17 gene sites and hypertension among the aboriginal Tibetan people[J]. Biomed Environ Sci, 2012, 25:526-532.
[3]	KIMURA L, ANGELI C B, AURICCHIO M T, et al. Multilocus family-based association analysis of seven candidate polymorphisms with essential hypertension in an Africanderived semi-isolated Brazilian population[J]. Int J Hypertens, 2012:859219.
[4]	GATTI R R, SANTOS P S, SENA A A, et al. The interaction of AGT and NOS3 gene polymorphisms with conventional risk factors increases predisposition to hypertension[J]. J Renin Angiotensin Aldosterone Syst, 2013, 14:360-368.
[5]	中国高血压防治指南修订委员会. 中国高血压防治指南(2010)[J]. 中华心血管病杂志, 2011,39(7):42-93.
[6]	张旺德, 赵孝梅, 何燕,等. 内皮型一氧化氮合酶基因rs3918181位点多态性与贵州汉族原发性高血压的关系[J]. 贵阳医学院学报, 2016, 41(1):8-11.
[7]	闫雪, 王伟, 毛用敏. 内皮型一氧化氮合酶基因rs3918181位点多态性与原发性高血压的关系研究[J]. 中国心血管杂志, 2013, 18(4):280-282.
[8]	YANG B, XU J R, LIU X M, et al. Polymorphisms of rs1799983(G>T) and rs1800780(A>G) of the eNOS gene associated with susceptibility to essential hypertension in the Chinese Hui ethic population[J]. Genet Mol Res, 2013, 12:3821-3829.
[9]	李凌,周婕,徐莉娜, 等. 贵州省城乡高血压病流行现状[J]. 中国健康教育, 2014, 30(5):407-410.
[10]	MIYAHARA K, KAWAMOTO T, SASE K, et al. Cloning and structural characterization of the human endothelial nitric oxide-synthase gene[J]. Eur J Biochem, 1994, 223:719-726.
[11]	李东宝, 华琦, 皮林. 内皮型一氧化氮基因G894T多态性与原发性高血压的相关关系[J]. 高血压杂志, 2004, 12(4):326-330.
[12]	王从, 孙刚, 闫旭龙, 等.蒙族高血压患者内皮型一氧化氮合酶(eNOS)基因多态性研究[J]. 中国分子心脏病学杂志, 2006, 6(2):81-84.
[13]	FERNANDEZ M L, RUIZ R, GONZALEZ M A. et al. Association of NOS3 gene with metabolic syndrome in hypertensive patients[J]. Thromd Haemost, 2004, 92:413-418.
[14]	李东宝,华琦,皮林. 内皮型一氧化氮合酶T786C多态性与原发性高血压的相关关系[J].首都医科大学学报, 2006, 27(2):226-229.
[15]	邹放君, 唐斌, 何芳, 等. 内皮型一氧化氮合酶基因T786C多态性与新疆汉族原发性高血压的相关性研究[J]. 中国慢性病预防与控制, 2011,19(2):114-117.
[16]	SEDIRI Y, KALLEL A, AYADI I, et al. Lack of association between endothelial nitric oxide synthase gene G894T polymorphism hypertension in the Tunisian population[J]. Prev Med, 2010, 51:88-89.
[17]	张强, 邓峰美, 何芳, 等. 新疆汉族和哈萨克族人群内皮型一氧化氮合成酶基因T786C和G894T多态性与原发性高血压的相关分析[J]. 中国动脉硬化杂志, 2012, 20(3):265-272.
[18]	周明, 党书毅, 王俊峰, 等. 中国汉族人群eNOS基因G894T多态性与原发性高血压关系的Meta分析[J]. 中国循证心血管医学杂志, 2012,10(4):408-411.
[19]	NEJATIZADEH A, KUMAR R, STOBDAN T, et al.Endothelial nitric oxide synthase gene haplotypes and circulating nitric oxide levels significantly associate with risk of essential hypertension[J]. Free Radi Biol Med, 2008, 44:1912-1918.
[20]	王刚, 唐斌, 何芳, 等. eNOS基因27bpVNTR多态性与新疆哈萨克族原发性高血压的相关性研究[J]. 石河子大学学报:自然科学版, 2010, 28(3):335-339.
[21]	方玲, 王柠, 林珉婷, 等. 皮型一氧化氮合酶基因第4内含子基因多态与福建汉族脑血管病的相关性[J]. 中国神经精神疾病杂志, 2011, 37(11):687-690.
[22]	高鹏,杜丹华,赵节绪,等.NOS基因多态性与脑卒中合并高血压关系的研究[J].中国老年学杂志,2008,28(22):2230-2231.