Antagonistic effect of perfluorocarbon emulsion on vasomotor activity factor of atherosclerosis rats
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摘要: 目的:使用高剪切乳化机制备全氟化碳乳剂 (PFCE), 通过静脉注射PFCE对动脉粥样硬化 (AS) 模型大鼠进行干预, 观察其内皮功能指标的变化, 初步探讨PFCE对AS大鼠血管舒缩活性因子的调控作用及对内皮的可能保护作用。方法:将50只Wistar雄鼠随机分为5组:空白对照组, 模型组, PFCE低、中、高剂量组, 每组10只。除空白对照组用普通维持饲料外, 其余均以维生素D3加高脂饲料喂养诱导实验性大鼠AS模型, PFCE低、中、高剂量组在造模同时给予不同剂量药物干预。12周后, 测定各组大鼠血清NO、ET-1、TXA2、6-K-PG的含量, 苏木精-伊红染色法观察各组大鼠主动脉病理变化。结果:模型组NO、TXA2含量显著降低, ET-1、6-K-PG含量显著升高, 与空白对照组比较差异有统计学意义 (P<0.05), 且NO/ET-1比值、TXA2/6-K-PG比值平衡失调;PFCE高、中剂量组较模型组血清NO、TXA2含量显著升高, ET-1、6-K-PG含量显著降低 (P<0.05), 并改善NO/ET-1及TXA2/6-K-PG的比值 (P<0.05);PFCE低剂量组与模型组相比血清中各指标差异无统计学意义 (P>0.05);PFCE高剂量组与PFCE低剂量组相比血清NO、TXA2含量显著升高, ET-1、6-K-PG含量显著降低 (P<0.05), 与中等剂量组差异不显著 (P>0.05);PFCE高、中剂量组较PFCE低剂量组减轻AS大鼠主动脉组织形态学变化更明显。结论:中等剂量以上PFCE可能通过调控AS大鼠血管舒缩活性因子, 起到保护血管内皮的功能, 发挥阻止AS进一步发生和发展的作用。Abstract: Objective:The perfluorocarbon emulsion was prepared by using a high shear emulsifier.The rats in the atherosclerotic model were treated by intravenous injection of PFCE to observe the changes of endothelial function.Method:Fifty Wistar males were randomly divided into 5 groups:blank control group, model group, PFCE low, medium and high dose group, with 10 rats in each group.In the control group, the experimental rats were induced by vitamin D3 plus high fat diet, and the treatment group was treated with different doses of drugs at the same time.After 12 weeks, the levels of serum NO, ET-1, TXA2 and 6-K-PG in each group were measured and the pathological changes of the aorta were observed by HE staining.Result:The content of NO and TXA2 in model group was significantly decreased (P<0.05), the content of ET-1, 6-K-PG was significantly increased, and the ratio of NO/ET-1 and TXA2/6-K-PG were significantly different from the control group (P<0.05).The levels of NO and TXA2 in the high and middle dose groups of PFCE were significantly higher than those in the model group, and the content of ET-1, 6-K-PG was significantly decreased and the ratio of NO/ET-1 and TXA2/6-KPG was improved (P<0.05).There was no significant difference in the serum levels between the low dose group and the model group (P>0.05).The levels of NO and TXA2 in the PFCE high dose group and the low dose group were significantly higher than those in the low dose group, and the content of ET-1, 6-K-And the difference was not significant in the middle dose group (P<0.05).The PFCE high and middle dose group reduced the morphological changes of the aorta in the AS group compared with the PFCE low dose group (P>0.05).Conclusion:Medium dose above perfluorocarbon emulsion may be regulated through the vasculature AS rats vasomotor activity factor, protect endothelial function, and play a role in preventing the occurrence and development of AS further.
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Key words:
- atherosclerosis /
- perfluorocarbon /
- vasomotor factors
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