The peripheral blood circularRNA profile in patients with ST-segment elevation myocardial infarction
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摘要: 目的:筛选急性ST段抬高型心肌梗死患者外周血差异表达的环状RNA, 探讨环状RNA在急性心肌梗死中的作用。方法:采用环状RNA芯片检测3例急性前壁心肌梗死和3例冠状动脉 (冠脉) 正常的健康对照者外周血中环状RNA的表达谱, 根据变化倍数和P值筛选差异表达的环状RNA, 通过生物信息学分析预测靶miRNA;对筛选出来的6个环状RNA, 采用荧光定量PCR方法检测其在急性ST段抬高型心肌梗死患者 (22例) 和正常对照患者 (21例) 外周血中的水平。结果:在882个环状RNA中, 急性前壁心肌梗死患者外周血中有460个 (52.2%) 表达上调, 422个 (47.8%) 表达下降;在上调的环状RNA中, 15个变化倍数＞10.0;在下调的环状RNA中, 14个变化倍数＞7.0。在急性ST段抬高型心肌梗死患者中对筛选出来的6个环状RNA进行检测, 环状RNA30741的外周血表达水平显著高于正常的健康对照者 (P＜0.01), 生物信息学分析显示环状RNA30741的靶miRNA是小分子RNA-21。结论:急性ST段抬高型心肌梗死患者外周血环状RNA的表达存在差异, 其中环状RNA30741可能参与了急性ST段抬高型心肌梗死的病理过程。Abstract: Objective: To detect the profile and function of circularRNA (circRNA) in patients with ST-segment elevation myocardial infarction (STEMI).Method: The peripheral blood samples from patients with STEMI (n=3) and normal coronary artery (n=3) were collected to investigate circRNA profile by circRNA chip.The difference of circRNA was identified according to change fold as well as Pvalue.Bioinformatics analysis was carried out to predict the target microRNA (miRNA) of circRNA.6 circRNAs were picked for further investigation in larger samples of patients with STEMI (n=22) and normal coronary artery (n=21).Result: 882 circRNAs were detected by circRNA chip.Among them, 460 circRNAs (52.2%) were up-regulated and 422 circRNAs (47.8%) were simultaneously down-regulated in STEMI patients.Change folds of 15 circRNAs in up-regulated circRNAs were more than 10.0, while change folds of 14 circRNAs in down-regulated circRNAs were more than 7.0.In 6 circRNAs picked for further investigation, the expression of circRNA30741 was found to significantly increased in STEMI patients (P＜0.01), while other 5 circRNAs did not significantly vary.miRNA-21 was predicted to be the target miRNA of circRNA30741.Conclusion: The different expression of circRNAs occurres in STEMI patients, circRNA30741 is related to STEMI.
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Key words:
- acute myocardial infarction /
- circular RNA /
- gene profile /
- target microRNA
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