压力负荷增高心肌多胺及肥大细胞变化研究

熊晓兰; 贾晓丽; 肖超; 倪情情; 张世忠

doi:10.13201/j.issn.1001-1439.2018.04.018

压力负荷增高心肌多胺及肥大细胞变化研究

- 三峡大学医学院(湖北宜昌, 443002)
基金项目:
国家自然科学基金 (No:30872716)

湖北省自然科学基金 (No:2015CFB288)

湖北省卫生厅科研项目 (No:WJ2015MB171)

三峡大学科研创新基金 (No:SDYC2016086)

详细信息

通讯作者: 张世忠,E-mail:zhangsz@ctgu.edu.cn

中图分类号: R542.2

收稿日期: 2017-09-18

Changes of myocardial polyamine content and mast cell after chronic pressure overload

- Medical Collgeg of China Three Gorges University, Yichang, 443002, China

More Information

Corresponding author: ZHANG Shizhong, E-mail: zhangsz@ctgu.edu.cn

Received Date: 18 September 2017

摘要

摘要: 目的:探讨慢性压力负荷增高性心肌肥厚大鼠心肌多胺含量及肥大细胞数目的变化规律。方法:通过缩窄大鼠腹主动脉制作压力负荷增高性心肌肥厚动物模型。分别于术后第1、2、3、4、5、6、7和8周称量大鼠体质量、心脏质量, 计算心质量指数;采用高效液相法测定心肌多胺含量;采用甲苯胺蓝染色观察心肌肥大细胞数目。结果:与假手术组大鼠比较, 手术组大鼠术后各周心指数明显增加 (P＜0.05), 左室心肌的多胺含量明显增高 (P＜0.05), 肥大细胞数量亦明显增多 (P＜0.05)。结论:慢性心脏压力负荷增高导致心肌肥厚的过程伴有多胺含量增高及肥大细胞数目增多。
- 慢性压力负荷过载 /
- 心肌重构 /
- 多胺 /
- 肥大细胞
Abstract: Objective: To investigate changes of myocardial polyamine content and mast cells count in cardiac hypertrophy induced by chronic pressure overload.Method: Rats of cardiac hypertrophy were induced by partial ligation of abdominal aorta.Body weight and heart weight were weighed at 1, 2, 3, 4, 5, 6, 7 and 8 th week after the operation.Heart weight index was calculated.Myocardial polyamine content was measured by high performance liquid method.The cardiac mast cells count was determined by Toluidine blue staining method.Result: Compared with rats in sham group, the heart weight index, the content of polyamine in myocardium and the count of cardiac mast cells all increased significantly every week after the operation in operation group (all P＜0.05).Conclusion: The cardiac chronic hypertrophy accompanies with increase of both cardiac polyamine content and mast cells count.
- chronic pressure overload /
- myocardium remodeling /
- polyamine /
- mast cells

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参考文献(14)

[1]	刘永政, 张双, 王楠, 等.运动康复治疗对合并肾功能不全的慢性心力衰竭患者心功能及生活质量的影响[J].临床心血管病杂志, 2017, 33 (1):54-57.
[2]	Moon TC, Befus AD, Kulka M.Mast cell mediators:their differential release and the secretory pathways involved[J].Front Immunol, 2014, 5:569.
[3]	Theoharides TC, Kempuraj D, Tagen M, et al.Differential release of mast cell mediators and the pathogenesis of inflammation[J].Immunol Rev, 2007, 217:65-78.
[4]	Garcia-Faroldi G, Rodriguez CE, Urdiales JL, et al.Polyamines are present in mast cell secretory granules and are important for granule homeostasis[J].PLoS One, 2010, 5 (11):e15071.
[5]	石海莲, 张蓓蓓, 刘燕, 等.左前肢血压法动态观察腹主动脉缩窄大鼠的血压变化[J].中国药理学通报, 2008, 24 (6):830-833.
[6]	任婷, 成细华, 陈聪, 等.多胺代谢与缺血性心血管疾病研究进展[J].湖南中医药大学学报, 2017, 37 (6):690-692.
[7]	林岩, 徐长庆, 赵雅君, 等.多胺在异丙肾上腺素所致大鼠心肌肥厚中的作用及意义[J].中国病理生理杂志, 2009, 25 (3):417-421.
[8]	刘宏伟, 姜冰, 万新, 等.大鼠局灶性脑缺血再灌注后PAO活性和多胺含量变化及意义[J].中南大学学报 (医学版), 2005, 30 (4):452-455.
[9]	Lin Y, Zhang X, Wang L, et al.Polyamine depletion attenuates isoproterenol-induced hypertrophy and endoplasmic reticulum stress in cardiomyocytes[J].Cell Physiol Biochem, 2014, 34 (5):1455-1465.
[10]	Tipnis UR, He GY, Li S, et al.Attenuation of isoproterenol-mediated myocardial injury in rat by an inhibitor of polyamine synthesis[J].Cardiovasc Pathol, 2000, 9 (5):273-280.
[11]	赵雅君, 张伟华, 王艳丽, 等.鸟氨酸脱羧酶/多胺系统在大鼠缺血预适应心肌保护中的作用[J].中国病理生理杂志, 2009, 25 (12):2295-2301.
[12]	李辉, 黄林枫, 文纯, 等.心脏肥大细胞及Toll样受体4在小鼠病毒性心肌炎中的作用[J].中国当代儿科杂志, 2013, 15 (10):896-902.
[13]	Shi GP, Bot I, Kovanen PT.Mast cells in human and experimental cardiometabolic diseases[J].Nat Rev Cardiol, 2015, 12 (11):643-658.
[14]	张光明, 崔炜.Kounis综合征[J].临床心血管病杂志, 2017, 33 (11):1040-1044.