冠心病患者CASP7基因启动子区域甲基化水平的研究

季慧慧; 郑中华; 陈晓敏; 段世伟

doi:10.13201/j.issn.1001-1439.2019.02.009

冠心病患者CASP7基因启动子区域甲基化水平的研究

- 1 宁波大学医学院, 浙江省病理生理学技术研究重点实验室(浙江宁波, 315211);
- 2 宁波市第一医院心血管中心
基金项目:
宁波大学王宽诚幸福基金

详细信息

通讯作者: 段世伟,E-mail:duanshiwei@nbu.edu.cn

中图分类号: 541541.4

收稿日期: 2018-09-26

Methylation level of CASP7 gene promoter in patients with coronary heart disease

- 1 Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo 315211, China;
- 2 Laboratory of Ningbo First Hospital and Cardiovascular Center of Ningbo First Hospital

More Information

Corresponding author: DUAN Shiwei, E-mail: duanshiwei@nbu.edu.cn

Received Date: 26 September 2018

摘要

摘要: 目的:研究CASP7基因启动子区域甲基化水平与冠心病 (CHD) 的关系。方法:将研究对象分为两组, 正常对照组 (571例) 和CHD组 (679例) 。用甲基化特异性实时定量聚合酶链技术 (qMSP) 检测CASP7基因启动子区域甲基化水平, 分析CASP7基因启动子区域甲基化水平与CHD的相关性。结果:与对照组比较, CHD组患者年龄、男性数目、2型糖尿病 (T2D) 及吸烟人数显著性增高, 差异具有统计学意义 (P＜0.05) 。比较两组间的生化指标发现, CHD患者的脂蛋白a[Lp (a) ]、C反应蛋白 (CRP) 、天冬氨酸转氨酶 (AST) 、碱性磷酸盐 (ALP) 及γ谷氨酰转肽酶 (γ-GT) 水平相较于对照组显著增高, 而HDL-C水平降低 (P＜0.05) 。此外, 在总体对照组及男性对照组中发现, CASP7基因启动子区域甲基化水平与年龄呈负相关 (P＜0.05) 。在CHD组中, CASP7基因启动子区域甲基化水平与Lp (a) 呈负相关 (P＜0.05) 。在男性T2D患者中, CASP7基因启动子区域甲基化水平与狭窄程度呈负相关 (P＜0.05) 。结论:CASP7基因启动子区域甲基化水平的上调很可能会对CHD产生保护作用。
- CASP7 /
- 冠心病 /
- DNA甲基化
Abstract: Objective:To study the relationship between the methylation level of the promoter region of CASP7 and coronary heart disease (CHD).Method:The subjects were divided into two groups, the normal control group (n=571) and CHD (n=679) group.The methylation level of CASP7 promoter was detected by the methylationspecific real-time quantitative polymerase chain reaction (qMSP), and the correlation between methylation level and CHD was studied.Result:Compared with normal control group, the CHD group had a greater number of males, type 2diabetes mellitus (T2D) patients, and smokers, all P＜0.05.In addition, the age of CHD was significantly older than normal controls, P＜0.05.Comparison of biochemical markers between two groups found that the levels of Lp (a), CRP, AST, ALP, andγ-GT in CHD patients were significantly higher than those in controls, while HDL levels were significantly lower, all P＜0.05.Besides, in total controls and male controls, the methylation level of CASP7 promoter region was negatively correlated with age, P＜0.05.In CHD patients, the methylation level of CASP7 promoter region was negatively correlated with Lp (a), P＜0.05.In male CHD patients with T2 D, methylation level of CASP7 promoter region was negatively correlated with the degree of stenosis, P＜0.05.Conclusion:Up-regulation of methylation level of CASP7 gene was likely to have a protective effect on CHD.
- CASP7 /
- coronary heart disease /
- DNA methylation

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