Correlation of miR-1, miR-133a, miR-208a and miR-499 with inflammatory factors and history of hypertension after coronary artery bypass grafting
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摘要: 目的：观察行冠状动脉旁路移植术（CABG）的患者血浆中microRNAs （miRs）的变化及其与炎性因子的相关性，并初步探讨高血压病史对其的影响。方法：收集心外科住院的26例急性冠脉综合征（ACS）患者，其中18例有高血压病史，采集所有患者术前24 h和术后24 h的外周静脉血6 ml，测定miRNAs和炎性因子水平。结果：①miR-1、miR-208a、miR-499分别与白细胞介素-6（IL-6）正相关；miR-1、miR-133a、miR-499分别与心肌肌钙蛋白T （cTnT）正相关，miR-1、miR-133a与肌酸激酶（CK）、肌酸激酶MM同工酶（CK-MM）正相关；手术前后，△miR-1、△miR-133a、△miR-499三者间正相关，与△miR-208a不存在相关性；△miR-208a与△IL-6相关（r=0.545，P=0.004）；②术后血浆miR-1、miR-133a、miR-208a、miR-499、IL-6、cTnT、CK、CK-MB、CK-MM的水平较术前显著升高（P<0.05）；③非高血压病史组△miR-133a和△miR-499高于高血压病史组，差异有统计学意义（P<0.05）。结论：①CABG术后心肌再灌注损伤及继发炎症伴随miR-1、miR-133a、miR-208a、miR-499水平升高，与cTnT、CK、CK-MM有一定的相关性，其中炎性因子IL-6的变化与miR-208a的变化有关；②高血压病史对行CABG术患者的miR-133a、miR-499的表达有一定影响。Abstract: Objective: To observe the changes of plasma microRNAs in patients undergoing coronary artery bypass grafting (CABG) and their correlation with inflammatory factors and to explore the effect of hypertension history.Method: A total of 26 acute coronary syndrome (ACS) patients admitted to cardiac surgery were collected, and 18 of them have hypertension history.The peripheral venous blood of all patients (6 ml) were collected 24 hours before operation and 24 hours after the operation.Then we detected the miRNAs and inflammatory factors.Result: ①miR-1, miR-208a and miR-499 were positively correlated with IL-6;miR-1, miR-133a and miR-499 were also correlated with cTnT.miR-1, miR-133a were positively correlated with CK and CK-MM.After operation, △miR-1, △miR-133a and △miR-499 were positively correlated with each other.△miR-208a was associated with △IL-6 (r=0.545, P=0.004);②The levels of miR-1, miR-133a, miR-208a, miR-499, IL-6, cTnT, CK, CK-MB, CK-MM were significantly increased after CABG surgery (P<0.05).③The levels of △miR-133a and △miR-499 in non-hypertension group were significantly higher than those in hypertension group (P<0.05).Conclusion: Myocardial reperfusion injury and secondary inflammation after CABG are accompanied by increased levels of miR-1, miR-133a, miR-208a and miR-499.The changes of inflammatory factor IL-6 are related to that of miR-208a.The history of hypertension has a certain influence on the expression of miR-133a and miR-499 in patients undergoing CABG.
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