新疆哈萨克族高血压患者血压水平与T淋巴细胞钙调神经磷酸酶及肿瘤坏死因子α的相关性研究

谭猛; 张源明; 孟小攀; 董建; 戴晨

doi:10.13201/j.issn.1001-1439.2019.12.015

新疆哈萨克族高血压患者血压水平与T淋巴细胞钙调神经磷酸酶及肿瘤坏死因子α的相关性研究

- 1 新疆医科大学第一附属医院心脏中心(乌鲁木齐, 830054);
- 2 新疆医科大学第一附属医院肾病科
基金项目:
国家自然科学基金项目(No:81760081)

详细信息

通讯作者: 张源明,E-mail:zymdxx@163.com

中图分类号: R544.1

收稿日期: 2019-06-28

Correlation among blood pressure level, calcineurin and tumor necrosis factor α in T lymphocyte in Xinjiang Kazakh patients with essential hypertension

- 1 Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China;
- 2 Department of Nephrology, First Affiliated Hospital of Xinjiang Medical University

More Information

Corresponding author: ZHANG Yuanming, E-mail: zymdxx@163.com

Received Date: 28 June 2019

摘要

摘要: 目的:研究新疆哈萨克族原发性高血压患者血压水平与外周血T淋巴细胞钙调神经磷酸酶(CaN)和肿瘤坏死因子α(TNF-α)表达情况的相关性。方法:随机选取2018-01-2019-06在新疆医科大学第一附属医院、达坂城社区保健站和乌鲁木齐市友谊医院就诊的未经降压药物治疗的120例哈萨克族原发性高血压患者,按最新高血压防治指南中高血压分级标准分为高血压1级组、高血压2级组与高血压3级组,另随机选取健康体检者作为对照组。每组40例,男女各20例。采集外周血并使用磁性激活细胞分选法(MACS)分离T淋巴细胞。分别运用实时荧光定量PCR和Western blot检测外周血T淋巴细胞CaN和TNF-αmRNA和蛋白表达水平。结果:与对照组相比,高血压2级组和高血压3级组T淋巴细胞CaN mRNA和蛋白以及TNF-αmRNA表达均提高(均P<0.05),高血压1级组与对照组相比无明显差异。Spearman秩相关性分析和有序多分类Logistic回归结果显示,CaN和TNF-α表达水平与血压水平呈正相关,高血压患者血压水平随着CaN和TNF-α表达增高而增高。结论:新疆哈萨克族原发性高血压患者血压水平随着CaN和TNF-α表达的增高而增高,CaN和TNF-α可能参与高血压发生发展过程。
- 高血压 /
- 哈萨克族 /
- T淋巴细胞 /
- 钙调神经磷酸酶 /
- 肿瘤坏死因子α
Abstract: Objective:To investigate the correlation among expression of calcineurin(CaN) and tumor necrosis factor α(TNF-α) in peripheral blood T lymphocytes and blood pressure in Xinjiang Kazakh patients with essential hypertension.Method:A total of 120 Kazakh patients with essential hypertension without anti-hypertensive drugs treatment from the First Affiliated Hospital of Xinjiang Medical University,Urumqi Friendship Hospital and Dabancheng Community Health Service Center from January 2018 to June 2019 were selected. According to the hypertension grading standard in the latest Hypertension Prevention and Treatment Guidelines,these patients were divided into hypertension group 1,hypertension group 2,and hypertension group 3. In addition,healthy subjects were selected as the control group.There were 20 males and 20 females in each group.Peripheral blood was collected and T lymphocytes were isolated using immunomagnetic cell sorting(MACS).The mRNA and protein expression of CaN and TNF-α in peripheral blood T lymphocytes were detected by real-time PCR and Western blot respectively.Result:Compared with control group,the mRNA and protein expressions of CaN and mRNA expression of TNF-α in hypertension group 2 and hypertension group 3 were significantly higher(all P<0.05),and there was no significant difference between hypertension group 1 and control group.Spearman rank correlation analysis and ordered multi-class Logistic regression analysis showed that the expression of CaN and TNF-α were positively correlated with blood pressure level,and the blood pressure level of hypertensive patients increased with the increase expression of CaN and TNF-α.Conclusion:The blood pressure level of Kazakh patients with essential hypertension increase with the increase expression of CaN and TNF-α.CaN and TNF-αmay be involved in the development of hypertension.
- hypertension /
- Kazakh /
- T lymphocytes /
- calcineurin /
- tumor necrosis factor α

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