Analyzing the endothelial microparticles of coronary artery blood and venous blood
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摘要: 目的:分析急性心肌梗死(AMI)患者冠状动脉(冠脉)血和外周血内皮细胞微粒(EMPs)水平,验证EMPs与急性心血管事件的相关性。方法:收集27例AMI患者和30例稳定性冠心病患者外周血,冠脉介入术中收集研究对象冠脉血标本。采用流式细胞技术对CD31+EMPs进行定性和定量分析。结果:AMI组患者冠脉血CD31+EMPs水平(11.93±5.13)%,外周静脉血(36.68±23.53)%,稳定性冠心病患者冠脉血CD31+EMPs水平(5.03±3.01)%,外周静脉血(17.84±12.78)%,两组差异具有统计学意义(P<0.001)。通过ROC曲线发现AMI中的外周血CD31+EMPS的最佳临界值为29.7%,灵敏度为0.59,特异性为0.83,约登指数为0.426,ROC曲线下的面积为0.733(95%CI 0.598~0.868)(P<0.05)。结论:AMI患者冠脉血及外周血EMPs水平均高于稳定性冠心病患者,提示血液中EMPs可能参与急性冠脉血栓事件,同时外周静脉血浆中EMPs水平可作为新的生物标志物之一。Abstract: Objective: To analyze endothelial microparticles(EMPs) levels in coronary and venous blood of patients with acute myocardial infarction(AMI).Methods: The 27 patients with AMI and 30 patients with stable coronary artery disease(SCAD) were collected. CD31+EMPs from venous and coronary blood were analyzed qualitatively and quantitatively by flow cytometry.Results: The CD31+EMPs level was(11.93±5.13)% in the coronary blood and(36.68±23.53)% in the venous blood in AMI group. The CD31+EMPs level was(5.03±3.01)% in the coronary blood and(17.84±12.78)% in the venous blood in SCAD group. The difference between the two groups was significant statistically(P<0.001). According to the ROC curve, the optimal critical value of EMPs CD31+in venous blood in AMI was 29.7%, the sensitivity was 0.59, the specificity was 0.83, the Yoden index was 0.426, and the area under the ROC curve was 0.733(95%CI0.598-0.868)(P<0.05).Conclusion: The EMPs levels of coronary and venous blood in patients with AMI are higher than patients with SCAD.
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Key words:
- AMI /
- EMPs /
- Coronary artery /
- Thrombus
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