Study on the correlation between ACE gene RS4343 polymorphism and atrial fibrillation in minority and Han populations in Guizhou Province
-
摘要: 目的:研究贵州省少数民族和汉族心房颤动(房颤)患者血管紧张素转化酶(ACE)基因rs4343多态性。方法:选取贵州地区医院房颤患者184例为病例组,其中少数民族51例,汉族133例。以年龄和性别为配对条件,选取同期贵州地区医院健康体检中心健康者226例为对照组,其中少数民族96例、汉族130例。采用聚合酶链式反应(PCR)、聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和基因测序方法检测两组人群的ACE基因rs4343基因型及等位基因分布特点。结果:健康对照组ACE基因rs4343多态性在少数民族组和汉族组中基因型分别以AA型和AG型为主,等位基因均以A基因为主;ACE基因rs4343型频率和等位基因频率在房颤组与健康对照组间差异显著(P<0.05),且房颤组G等位基因频率显著高于健康对照组,为健康对照组的1.79倍;少数民族房颤患者ACE基因rs4343以AA型为主,汉族房颤患者ACE基因rs4343以AG型为主,等位基因均以A型为主,基因型频率和等位基因频率差异不具统计学意义。ACE基因rs4343型频率和等位基因频率在少数民族组和汉族组间差异无统计学意义。结论:ACE基因rs4343多态性与房颤显著相关,G等位基因是房颤发生的危险因子。
-
关键词:
- 心房颤动 /
- 血管紧张素转化酶基因rs4343 /
- 贵州省 /
- 少数民族 /
- 基因多态性
Abstract: Objective: To explore the polymorphism of angiotensin converting enzyme RS4343 gene in patients with atrial fibrillation(AF) of ethnic minorities and Han nationality in Guizhou province.Methods: A total of 184 patients with AF in Guizhou were selected as the case group, including 51 patients with ethnic minorities and 133 patients conditions, and 226 healthy patients were selected as control group, including 96 patients with ethnic minorities and 130 patients with Han nationality. Polymerase chain reaction(PCR), PCR-restriction fragment length polymorphism(PCR-RFLP) and gene sequencing were used to detect the distribution Characteristics of ACE gene RS4343.Results: The genotypes of ACE gene rs4343 were AA and AG, and the alleles were A, and there was no significant difference in genotype frequency and allele frequency(P>0.05). The polymorphism of ACE gene RS4343 in healthy control group was dominated by AA and AG genotypes respectively in minority group and Han group, and alleles were dominated by A gene. The genotype frequency and allele frequency of ACE gene RS4343 were significantly different between the atrial fibrillation group was significantly higher than that of the healthy control group(1.79 times).Conclusion: The polymorphism of ACE gen RS4343 is significantly correlated with AF, and G allele is risk factor of AF.-
Key words:
- atrial fibrillation /
- ACErs4343 /
- Guizhou province /
- minority /
- gene polymorphism
-
[1] Huang HD,Pietrasik G,Abid QU,et al.Supplemental radiofrequency ablation after acutely unsuccessful cryoballoon pulmonary vein isolation is associated with increased risk of recurrent atrial fibrillation[J].J Am Heart Assoc,2020,9(10):e015979.
[2] Oliver P,Guttman N,Menelao S,et al.Predictors of atrial fibrillation in hypertrophic cardiomyopathy[J].Heart,2017,33(10):110.
[3] Rowin EJ,Hausvater A,Link MS,et al.Clinical profile and consequences of atrial fibrillation in hypertrophic cardiomyopathy[J].Circulation,2017,136(25):2420-2436.
[4] Li Hua,Li Changsheng,et al.The prevalence,incidence,management and risks of atrial fibrillation in an elderly Chinese population:a prospective study[J].BMC Cardiovasc Disord,2015,35(12):110.
[5] Zhang W,Wang JG.Prevention of atrial fibrillation by intensive antihypertensive treatment[J].Hypertension,2020,75(6):1414-1416.
[6] Zhang J,Huang X,Wang X,et al.Identification of potential crucial genes in atrial fibrillation:a bioinformatic analysis[J].BMC Med Genomics,2020,13(1):104.
[7] Chen B,You X,Lin Y,et al.A systematic review and meta-analysis of the effects of early mobilization therapy in patients after cardiac surgery:A protocol for systematic review[J].Medicine(Baltimore),2020,99(4):e18843.
[8] Choi KH,Kim JH,Lee C,et al.Microbleeds and outcome in patients with acute ischemic stroke and atrial fibrillation taking anticoagulants[J].Stroke,2020,51(12):3514-3522.
[9] Lei N,Kareem M,Moon SK,et al.Hybrid decision support to monitor atrial fibrillation for stroke prevention[J].Int J Environ Res Public Health,2021,18(2):813.
[10] 赵子明,袁义强,尹晓盟,等.持续性心房颤动射频消融术后抗心律失常药物应用时程与预后的相关性分析[J].临床心血管病杂志,2019,35(1):60-64.
[11] Puertas RD,Arora R,Rome S,et al.Epigenetics in atrial fibrillation:a reappraisal[J].Heart Rhythm,2021,24:150.
[12] Feng W,Sun L,Qu X.Association of AGTR1 and ACE2 gene polymorphisms with structural atrial fibrillation in a Chinese Han population[J].Pharmazie,2017,72(1):17-21.
[13] Achouri-Rassas A,Ali NB,Cherif A,et al.Association between ACE polymorphism,cognitive phenotype and APOE E4 allele in a Tunisian population with Alzheimer disease[J].J Neural Transm(Vienna),2016,123(3):317-321.
[14] Ueberham L,Bollmann A,Shoemaker MB,et al.Genetic ACE I/D polymorphism and recurrence of atrial fibrillation after catheter ablation[J].Circ Arrhythm Electrophysiol,2013,6(4):732-737.
[15] Ma R,Li X,Su G,et al.Angiotensin-converting enzyme insertion/deletion gene polymorphisms associated with risk of atrial fibrillation:A meta-analysis of 23 case-control studies[J].J Renin Angiotensin Aldosterone Syst,2015,16(4):793-793.
[16] Atie H,Do A,Emra N,et al.ACE gene rs4343 polymorphism elevates the risk of preeclampsia in pregnant women[J].J Hum Hypertens,2018,33(4):120.
[17] 邓炎尧.ACE基因位点多态性与阿尔茨海默病的相关性研究[J].南方医科大学学报,2015,9:1325-1330.
[18] Anderson ME.When will we know enough to treat atrial fibrillation[J].Heart Rhythm,2007,4(6):750-761.
[19] Liu T,Korantzopoulos P,Xu G,et al.Association between angiotensin-converting enzyme insertion/deletion gene polymorphism and atrial fibrillation:a meta-analysis[J].Europace,2011,13(3):346-354.
[20] Tziakas DN,Chalikias GK,Stakos DA,et al.Effect of angiotensin-converting enzyme insertion/deletion genotype on collagen type I synthesis and degradation in patients with atrial fibrillation and arterial hypertension[J].Expert Opin Pharmacother,2007,8(14):2225-2234.
[21] Kuskaeva AV,Niculina SY,Chernova AA,et al,Cherkashina II.The role of the I/D polymorphism of the ACE gene in the development of atrial fibrillation[J].Kardiologiia,2018,10(2):5-9.
[22] 王亚珠,李瑛,范晋奇,等.血管紧张素转换酶基因rs4340和rs4343多态性与心房颤动的相关性[J].中国生物制品学杂志,2012,25(9):1198-1202.
[23] Pan M,Jiang MH,Wei MF,et al.Association of angiotensin-converting enzyme gene 2350G>A polymorphism with myocardial infarction in a Chinese population[J].Clin Appl Thromb Hemost,2009,15(4):435-442.
[24] Ravn LS,Benn M,Nordestgaard BG,et al.Angiotensinogen and ACE gene polymorphisms and risk of atrial fibrillation in the general population[J].Pharmacogenet Genomics,2008,18(6):525-533.
[25] Kuskaeva AV,Niculina SY,Chernova AA,et al.The role of the I/D polymorphism of the ACE Gene in the development of atrial fibrillation[J].Kardiologiia,2018,11(2):5-9.
[26] Ma R,Li X,Su G,et al.Angiotensin-converting enzyme insertion/deletion gene polymorphisms associated with risk of atrial fibrillation:A meta-analysis of 23 case-control studies[J].J Renin Angiotensin Aldosterone Syst,2015,16(4):793-800.
[27] 朱文青,陈庆兴.《2020ESC/EACTS心房颤动诊断与管理指南》更新解读[J].临床心血管病杂志,2020,36(11):975-977.
[28] Liang S,Pan M,Hu N,et al.Association of angiotensin-converting enzyme gene 2350 G/A polymorphism with diabetic retinopathy in Chinese Han population[J].Mol Biol Rep,2013,11:140.
[29] Sohail H,Hassan SM,Yaqoob U,et al.The height as an independent risk factor of atrial fibrillation:A review[J].Indian Heart J,2021,73(1):22-25.
[30] Zhang XL,Wu LQ,Liu X,et al.Association of angiotensin-converting enzyme gene I/D and CYP11B2 gene-344T/C polymorphisms with lone atrial fibrillation and its recurrence after catheter ablation[J].Exp Ther Med,2012,4(4):741-747.
[31] Xiao P,Ling Z,Woo K,et al.Renin-angiotensin system-related gene polymorphisms are associated with risk of atrial fibrillation[J].Am Heart J,2010,160(3):496-505.
[32] Xiao HD,Fuchs S,Campbell DJ,et al.Mice with cardiac-restricted angiotensin-converting enzyme(ACE)have atrial enlargement,cardiac arrhythmia,and sudden death[J].Am J Pathol,2004,165(3):1019-1032.
[33] Tsai CT,Lai LP,Lin JL,et al.Renin-angiotensin system gene polymorphisms and atrial fibrillation[J].Circulation,2004,109(13):1640-1646.
[34] Bahramali E,Rajabi M,Jamshidi J,et al.Association of ACE gene D polymorphism with left ventricular hypertrophy in patients with diastolic heart failure:a case-control study[J].BMJ Open,2016,6(2):e010282.
[35] Simes SC,Balico-Silva AL,Parreiras-E-Silva LT,et al.Signal transduction profiling of angiotensin II Type 1 receptor with mutations associated to atrial fibrillation in humans[J].Front Pharmacol,2020,11:150.
[36] 祁伶姗,赵珺,李健,等.血管紧张素-(1-7)及其信号转导途径拮抗剂对犬急性心房电重构的影响[J].中国心脏起搏与心电生理杂志,2014,28(2):151-154.
[37] Lu X,Chen G,Yang Y,et al.Angiotensin-converting enzyme polymorphism affects outcome of local Chinese with acute lung injury[J].Respir Med,2011,105(10):150-160.
[38] Wei F,Lin S,Xiu FQ.Association of AGTR1 and ACE2 gene polymorphisms with structural atrial fibrillation in a Chinese Han population[J].Pharmazie,2017,72(1):160-170.
计量
- 文章访问数: 484
- PDF下载数: 119
- 施引文献: 0