L-茶氨酸抑制P53/P16改善D-半乳糖诱导的人脐静脉内皮细胞和心肌细胞损伤

刘敏; 刘晓峰; 张苏川; 姚峰; 王莉; 朱磊; 程波; 杨明; 吴虹; 黄璐; 戴婷; 代天; 刘建华

doi:10.13201/j.issn.1001-1439.2020.01.017

L-茶氨酸抑制P53/P16改善D-半乳糖诱导的人脐静脉内皮细胞和心肌细胞损伤

- 1. 江汉大学附属医院(武汉, 430030);
- 2. 汉口医院
基金项目:
武汉市卫健委科研项目(No:WX18D24)

详细信息

通讯作者: 刘建华,E-mail:376219789@qq.com

中图分类号: R541.7

收稿日期: 2019-11-19

L-theanine protects against D-galactose-induced injury of human umbilical vein endothelial cells and cardiomyocytes via inhibiting P53/P16

- 1. Affiliated Hospital of Jianghan University, Wuhan, 430030, China;
- 2. Hankou Hospital

More Information

Corresponding author: LIU Jianhua, E-mail: 376219789@qq.com

Received Date: 19 November 2019

摘要

摘要: 目的:探讨L-茶氨酸对D-半乳糖诱导人脐静脉内皮细胞(HUVECs)和心肌细胞(H9c2)损伤的保护作用。方法:通过D-半乳糖诱导HUVECs和H9c2细胞损伤。采用CCK8法检测细胞存活率,分光光度法检测细胞SOD酶活力和MDA含量,western blot测定细胞P53、P16、Rb和磷酸化Rb蛋白表达,评价L-茶氨酸的保护作用和可能机制。结果:L-茶氨酸(5和10 mmol/L)显著性抑制D-半乳糖诱导的HUVECs和H9c2凋亡,升高细胞SOD酶活力,降低MDA含量,抑制P53和P16表达并提高Rb的磷酸化水平。结论:L-茶氨酸能够保护D-半乳糖诱导HUVECs和H9c2损伤,其机制与调控P53和P16通路有关。
- L-茶氨酸 /
- D-半乳糖 /
- 人脐静脉内皮细胞 /
- 心肌细胞
Abstract: Objective:To investigate the protective effects of L-theanine against D-galactose-induced injury of human umbilical vein endothelial cells(HUVECs) and cardiomyocytes(H9c2).Method:The cells injure model was induced by D-galactose.The protective effects of L-theanine and its possible mechanisms were evaluated by measuring the cell survival rate,the activity of SOD and the content of MDA in cell as well as the expression of P53,P16,Rb and phosphorylated Rb.Result:L-theanine(5 and 10 mmol/L) significantly inhibited the apoptosis of HUVECs and H9c2,increased the activity of SOD,decreased the level of MDA,inhibited the expression of P53 and P16,as well as increased the phosphorylation level of Rb induced by D-galactose.Conclusion:L-theanine can protect against D-galactose induced injury of HUVECs and H9c2 via inhibiting P53 and P16 pathway.
- L-theanine /
- D-galactose /
- Human umbilical vein endothelial cells /
- Cardiomyocytes

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参考文献(15)

[1]	Mathew John C,Khaddaj Mallat R,Mishra RC,et al.SKA-31,an activator of Ca²⁺-activated K⁺ channels,improves cardiovascular function in aging[J].Pharmacol Res,2019,doi:10.1016/j.phrs.2019.104539.
[2]	吴洪渊,王媛媛,董合玲,等.CARTPT对过氧化氢诱导人脐静脉内皮细胞衰老的影响[J].广东医学,2018,39(14):2117-2121.
[3]	Mongiardi MP,Merolle M,Fustaino V,et al.Gene expression profiling of hypoxic response in different models of senescent endothelial cells[J].Aging Clin Exp Res,2019,doi:10.1007/s40520-019-01390-5.
[4]	Salcan S,Bongardt S,Monteiro Barbosa D,et al.Elastic titin properties and protein quality control in the aging heart[J].Biochim Biophys Acta Mol Cell Res,2019,doi:10.1016/j.bbamcr.2019.118532.
[5]	张宇,张新建,郭美华,等.姜黄素抗大鼠心肌细胞衰老的作用及机制[J].中国药师,2019,22(9):1629-1633.
[6]	马四补,刘英,田兴中,等.红景天破壁饮片与传统饮片对缺血缺氧H9c2心肌细胞保护作用对比研究[J].亚太传统医药,2019,7(15):18-23.
[7]	Alagawany M,Abd El-hack ME,Saeed M,et al.Nutritional applications and beneficial health applications of green tea and L-theanine in some animal species:A review[J].J Anim Physiol Anim Nutr (Berl),2019,doi:10.1111/jpn.13219.
[8]	赵紫伟.衰老及抗衰老药物信号通路及发展前景[J].中西医结合心血管病杂志,2018,6(25):26-26.
[9]	王烙佩,郑杰,李思敏,等.自噬在心脏衰老过程中的作用及其调控机制研究进展[J].巴楚医学,2019,1(2):107-110.
[10]	段丽,李聪,罗悦,等.原花青素B2通过GATA4/p53通路改善肝细胞衰老的研究[J].现代食品科技,2019,35(8):1-5.
[11]	苗新宇,朱潇潇,龚燕平,等.原代大鼠主动脉内皮细胞衰老模型的建立[J].细胞与分子免疫学杂志,2019,35(3):230-235.
[12]	高亮亮,肖建如.P53基因在小鼠衰老中的调控作用研究[J].基因组学与应用生物学,2018,37(6):2663-2667.
[13]	周勇,刘晶,陈刚.淫羊藿苷对D-半乳糖诱导的脑衰老模型小鼠P53/P21信号通路的影响[J].中华中医药学刊,2019,37(9):2159-2162.
[14]	吴飞翔,陈俞翰,李邵琦,等.多胺代谢与自噬在增龄大鼠心脏中的变化及外源性精脒对衰老心脏自噬的影响[J].中国药理学通报,2019,35(8):1073-1079.
[15]	苏路路,管博文,樊飞跃,等.衰老细胞清除剂研究进展.中国药理学通报,2019,35(10):1333-1337.