白细胞介素-17对大鼠心肌梗死后室性心律失常的影响

陈昭喆; 陈志坚; 曾秋棠; 刘学刚; 张青; 刘立新; 朱云涛; 钱程; 杜以梅; 赵宁

doi:10.13201/j.issn.1001-1439.2012.06.013

白细胞介素-17对大鼠心肌梗死后室性心律失常的影响

- 华中科技大学协和医院心内科华中科技大学同济医学院心血管病研究所湖北省生物靶向治疗研究重点实验室（武汉，430022）
基金项目:
国家重点基础发展研究973计划(No:2007CB512005)

国家自然科学基金项目(No:30770880/30971242)

详细信息

通讯作者: 陈志坚,E-mail:drchenok@sohu.com

中图分类号: R542.2

收稿日期: 2011-11-30

修回日期: 2012-02-10

Effect of IL-17 expression on ventricular arrhythmias in post-myocardial infarction rats

- Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China

More Information

Corresponding author: CHEN Zhijian, E-mail: drchenok@sohu.com

Received Date: 30 November 2011

Revised Date: 10 February 2012

摘要

摘要: 目的:探讨大鼠心肌梗死(MI)后白细胞介素-17(IL-17)在室性心律失常发生中的作用。方法:采用液氮冷冻法建立大鼠MI模型共138只,随机分为MI对照组(MI组)、小鼠同型IgG组(IgG组)和IL-17抗体组。每组又随机分为3个亚组:2周组、4周组及8周组。另设假手术组。假手术组和MI组分别腹腔注射0.8mlPBS溶液,并注入0.2ml空气促使液体分散到腹腔内;IgG组、IL-17抗体组分别注射小鼠同型IgG1+PBS溶液(100μg IgG1溶于0.8ml PBS,同样注入0.2ml空气)、小鼠抗人IL-17单克隆抗体+PBS溶液(100μg IL-17抗体溶于0.8ml PBS溶液,同样注入0.2ml空气)。每隔1d用药1次,连续用药5次。运用程序电刺激方法,观察各组室性心律失常的诱发率,同时用Western blot和激光共聚焦显微镜检测IL-17蛋白表达水平。结果:与假手术组比较,MI组大鼠心肌组织IL-17表达显著增加(P<0.05),室性心律失常诱发率亦明显增高(P<0.05);与MI组比较,IL-17抗体组IL-17表达显著减少(P<0.05),室性心律失常诱发率亦明显降低(P<0.05)。大鼠心肌IL-17的表达水平和室性心律失常诱发率之间呈正相关性(r=0.88,P<0.05),但IgG组与MI组比较差异无统计学意义(P>0.05)。结论:MI后大鼠心肌组织IL-17表达明显增加,大量IL-17表达与MI后室性心律失常的发生密切相关。IL-17的大量表达可能对MI后室性心率失常折返环的形成有诱导或促进作用。
- 心肌梗死 /
- 白细胞介素-17 /
- 室性心律失常
Abstract: Objective:To investigate the effect of IL-17 expression on ventricular arrhythmias in rats with myocardial infarction(MI). Method:Liquid nitrogen cryoinjury method was used in rats to establish MI model.The MI model rats were randomly divided into 3 groups,MI group,IgG group and IL-17 antibady group.Then each group was randomly divided into 3 sub-groups: 2 week,4 week and 8 week group.Sham group was established.The first day after operation,PBS solution were intraperitoneally injected to sham group and MI group,mouse isotype IgG1 and PBS to IgG group,monoclonal human IL-17 antibody and PBS to IL-17 antibady group,5 times every 2 days.Programmed electrical stimulus was imposed on the rat hearts to induce ventricular arrhythmia(ventricular tachycardia and ventricular fibrillation).The evoked electrocardiograms and evoked rates were recorded.The protein expression of IL-17 was examined by Western blot and LSCM. Result:Compared to sham group,expression of IL-17 in myocardium in MI group,IgG group and IL-17 antibody group increased obviously(P<0.05) at 2 weeks、4 weeks and 8 weeks after operation.The evoked rates of ventricular arrhythmia also increased(P<0.05).Compared to MI group and IgG group,expression of IL-17 in IL-17 group was lower(P<0.05),and the evoked rates decreased too(P<0.05).While,obvious differences were not found between MI group and IgG group.Statistic analysis showed that there was a positive linear correlation between expression of IL-17 and theevoked rate(r=+0.88,P<0.05). Conclusion:IL-17 expression significantly increased after MI.There is a relationship between a large number of IL-17 expression and ventricular arrhythmias in rats with MI.High expression of IL-17 may induce or promote the formation of the reentrant circuit of ventricular arrhythmias in post-myocardial infarction rats.
- myocardial infarction /
- IL-17 /
- ventricular arrhythmia

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