白细胞介素-27在小鼠病毒性心肌炎中的表达

黄凯; 孔清; 潘晓芬; 赖文盈; 石昌荆; 伍伟锋

doi:10.13201/j.issn.1001-1439.2014.02.008

白细胞介素-27在小鼠病毒性心肌炎中的表达

- 广西医科大学第一附属医院心内科广西心血管病研究所(南宁, 530021)
基金项目:
国家自然科学基金资助项目 No:81160032)

详细信息

通讯作者: 伍伟锋,E-mail:wucna65@163.com

中图分类号: R542.2

收稿日期: 2013-08-24

修回日期: 2013-10-22

Expressions of IL-27 in the coxsackievirus B3-induced mice viral myocarditis

- Department of Cardiology, the First Affiliated Hospital of Guangxi Medical University, Guangxi Cardiovascular Institute, Nanning, 530021, China

More Information

Corresponding author: WU Weifeng, E-mail: wucna65@163.com

Received Date: 24 August 2013

Revised Date: 22 October 2013

摘要

摘要: 目的:研究小鼠病毒性心肌炎 (VMC) 模型中白细胞介素 (IL) -27基因和蛋白的表达, 探究其在VMC发病中的作用及意义。方法:VMC组用柯萨奇病毒B3 (CVB3) 感染Balb/c小鼠建立VMC模型, 注射100TCID 50病毒液0.1ml;对照组注射等量磷酸盐缓冲液 (PBS)。在注射后的第0、1、2、3、4和6周应用逆转录-聚合酶链反应检测两组小鼠心肌组织中IL-27亚基p28及EBI3的mRNA表达, 酶联免疫双夹心抗体法检测血清中IL-27蛋白的表达。结果:与对照组比较, VMC组小鼠心肌组织中IL-27p28mRNA水平自第1周开始升高, 第2周时达峰值, 并至少维持至第6周 (均P<0.05);VMC组第1⁶周各时点血清IL-27蛋白均明显高于对照组 (均P<0.05);与对照组同时点比较, VMC组各时点小鼠心肌组织中IL-27EBI3mRNA差异均无统计学意义。结论:IL-27在VMC小鼠中高表达, 提示IL-27可能参与VMC的发病机制。
- 病毒性心肌炎 /
- 白细胞介素-27
Abstract: Objective: To investigate the expression of interleukin-27 (IL-27) in the coxsackievirus B3-induced mice viral myocarditis (VMC).Method: BALB/c mice were intraperitoneally infected with CVB 3for establishing VMCmodels.Control mice were treated with phosphate-buffered saline (PBS).On 0, 1, 2, 3, 4, 6week after injection, expressions of serum IL-27were analyzed by enzyme linked immosorbent assay (ELISA).Productions of cardiac IL-27p28and EBI3mRNA were measured by real time-polymerase chain reaction (RT-PCR).Result: Levels of cardiac IL-27p28mRNA obviously increased in VMC mice on week 1after infection, peaked on week 2, and highly persisted to at least week 6 (all P<0.05).Compared with control group, serum IL-27protein was higher in VMC group from week 1to week 6 (all P<0.05).There was no significant difference production of cardiac IL-27EBI3mRNA was found between VMC and control group throughout the course of the experiment.Conclusion: The increased levels of IL-27may play an important role in the pathogenesis of CVB3-induced mice VMC.
- viral myocarditis /
- interleukin-27

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