结缔组织生长因子通过增强Caspase-3活性调节ox-LDL诱导THP-1细胞凋亡的机制

周心涛; 赵黎丙; 郎明健

doi:10.13201/j.issn.1001-1439.2014.06.021

结缔组织生长因子通过增强Caspase-3活性调节ox-LDL诱导THP-1细胞凋亡的机制

- 湖北医药学院附属东风医院心内科(湖北十堰, 442000)
基金项目:
湖北省自然科学基金重点项目 (No:2009-2060402-791)

详细信息

通讯作者: 郎明健:E-mail:lmjian1976@sohu.com

中图分类号: R534.1

收稿日期: 2013-12-13

Mechanism of connective tissue growth factor enhance Caspase-3 activity to regulate ox-LDL induced THP-1 apoptosis

- Department of Cardiology, Dongfeng Hospital Hubei Medical College, Shiyan, Hubei, 442000, China

More Information

Corresponding author: LANG Mingjian, E-mail: lmjian1976@sohu.com

Received Date: 13 December 2013

摘要

摘要: 目的:研究动脉粥样硬化过程中结缔组织生长因子 (CTGF) 在巨噬细胞凋亡过程中发挥的作用及机制。方法:对人巨噬细胞 (THP-1) 分组进行干预, 采用SiRNA基因沉默方法, 对CTGF基因进行靶向沉默, 用细胞流式、实时定量、蛋白印迹及酶活性检测等技术, 阐明CTGF在氧化性低密度脂蛋白 (ox-LDL) 诱导THP-1凋亡过程中起的作用及机制。结果:CTGF在ox-LDL刺激THP-1细胞组高表达, 而CTGF靶向性SiRNA可以降低其表达;沉默CTGF后, THP-1凋亡率明显降低, 并且与Caspase-3酶活性呈正相关。结论:内源性的CTGF可以通过增强Capsase-3酶活性来促进THP-1的凋亡。
- 结缔组织生长因子 /
- 细胞凋亡 /
- Caspase-3
Abstract: Objective: To research the role and mechanism of connective tissue growth factor (CTGF) during process of the macrophage apoptosis.Method: We stimulated the different group of the human macrophage (THP-1).We used the RNA interfering technology to influence gene of CTGF, and observed the role and mechanism of CTGF during process of the THP-1apoptosis, which were detected by flow cytometry, PCR, Western blot, and ELISA.Result: Ox-LDL induced the high expression of CTGF in THP-1cells, and the SiRNA of CTGF decreased the expression of CTGF, this effect also influenced the apoptosis rate of THP-1cells.In addition, the activation of Caspase-3also changed with the SiRNA of CTGF.Conclusion: Endogenous CTGF can enhance the activity of Caspase-3to induce the THP-1cells apoptosis.
- connective tissue /
- growth factor /
- apoptosis

HTML全文

参考文献(4)

[1]	VAN VRE E A, AIT-OUFELLA H, TEDGUI A, et al.Apoptotic cell death and efferocytosis in atherosclerosis[J].Arterioscler Thromb Vasc Biol, 2012, 32:887-893.
[2]	ROSSI M L, MARZILIANO N, MERLINI P A, et al.Different quantitative apoptotic traits in coronary atherosclerotic plaques from patients with stable angina pectoris and acute coronary syndromes[J].Circulation, 2004, 110:1767-1773.
[3]	方丽娟, 刘乃丰.细胞凋亡与动脉粥样硬化关系的研究进展[J].东南大学学报 (医学版), 2010, 29 (1):107
[4]	OEMAR B S, WERNER A, GARNIER J M, et al.Human connective tissue growth factor is expressed in advanced atherosclerotic lesions[J].Circulation, 1997, 95:831-839.