新疆维吾尔族、汉族新发心房颤动患者microRNA-328的表达水平

方志敏; 吴庆; 陆云; 刘顺民; 赵海燕; 钟小兰; 宋宏雁; 班努·库肯; 刘超群

doi:10.13201/j.issn.1001-1439.2016.03.004

新疆维吾尔族、汉族新发心房颤动患者microRNA-328的表达水平

- 新疆医科大学第二附属医院心内科(乌鲁木齐, 830063)

详细信息

通讯作者: 方志敏,E-mail:fangzhimin15@sina.com

中图分类号: R541.7

收稿日期: 2015-08-27

Expression of microRNA-328 in patients with new onset atrial fibrillation of Uygur and Han nationality in Xinjiang

- Department of Cardiology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830063, China

More Information

Corresponding author: FANG Zhimin, E-mail: fangzhimin15@sina.com

Received Date: 27 August 2015

摘要

摘要: 目的:比较microRNA-328在新疆维吾尔族及汉族新发心房颤动(AF)患者和窦性心律者中的表达水平。方法:收集2014-01-2015-06在新疆医科大学第二附属医院新发AF患者37例的病历资料,采用实时荧光定量聚合酶链反应(qRT-PCR)检测microRNA-328,比较血浆中的microRNA-328表达水平。结果:窦性心律组和新发AF组microRNA-328表达水平差异有统计学意义(P＜0.01)。将维吾尔族和汉族作为协变量分层,比较窦性心律组和新发AF组microRNA-328表达水平的差异,结果显示维吾尔族、汉族microRNA-328表达在窦性心律组和新发AF组中均差异有统计学意义(P＜0.01),不同民族表达具有一致性。结论:新发AF患者血浆microRNA-328表达水平增高,无论在维吾尔族或汉族患者中均具有一致性,提示microRNA-328可作为新发AF的生物标志物。
- 心房颤动 /
- microRNA-328 /
- 聚合酶链反应
Abstract: Objective:To compare the expression level of microRNA-328 in Xinjiang Uygur and Han patients with new onset atrial fibrillation (AF) and sinus rhythm.Method:A total of 37 cases with new AF in the Second Affiliated Hospital of Xinjiang Medical University from January 2014 to June 2015 were included. Real-time fluorescence quantitative polymerase chain reaction (QRTPCR) was used to detect microRNA-328 plasma expression level.Result:There was significant difference in microRNA-328 expression between sinus rhythm group and AF group (P<0.05). The expressions of microRNA-328 in sinus rhythm group and AF group were statistically significant both in Uygur and Han patients (P<0.05).Conclusion:The plasma expression level of microRNA-328 is increased in new AF both in Uygur and Han patients, suggesting that microRNA-328 can be used as a biological marker of new onset AF.
- atrial fibrillation /
- microRNA-328 /
- polymerase chain reaction

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参考文献(18)

[1]	BENJAMIN E J,CHEN P S,BILD D E,et al.Prevention of atrial fibrillation:report from a national heart,lung,and blood institute workshop[J].Circulation,2009,119:606-618.
[2]	MIYASAKA Y,BARNES M E,GERSH B J,et al.Secular trends in incidence of atrial fibrillation in Olmsted County,Minnesota,1980to 2000,and implications on the projections for future prevalence[J].Circulation,2006,114:119-125.
[3]	周自强,胡大一,陈捷,等.“中国心房颤动流行病学研究”结果解读[J].中华内科杂志,2010,49(3):198-199.
[4]	VAZIRI S M,LARSON M G,BENJAMIN E J,et al.Echocardiographic predictors of nonrheumatic atrial fibrillation.The Framingham Heart Study[J].Circulation,1994,89:724-730.
[5]	LU Y,ZHANG Y,WANG N,et al.Micro RNA-328 Contributes to Adverse Electrical Remodeling in Atrial Fibrillation[J].Circulation,2010,122:2378-2387.
[6]	GILAD S,MEIRI E,YOGEV Y,et al.Serum microRNAs are promising novel biomarkers[J].PLoS One,2008,5,3:e3148.
[7]	BARBEAU E J,TAYLOR M J,REGIS J,et al.Spatio temporal dynamics of face recognition[J].Cereb Cortex,2008,18:997-1009.
[8]	CHIM S S,SHING T K,HUNG E C,et al.Detection and characterization of placental microRNAs in maternal plasma[J].Clin Chem,2008,54:482-490.
[9]	MITCHELL P S,PARKIN R K,KROH E M,et al.Circulating micro-RNAs as stable blood-based markers for cancer detection[J].Proc Nat Acid Sci USA,2008,105:10513-10518.
[10]	WIJFFELS M C,KIRCHHOF C J,DORLAND R,et al.Atrial fibrillation begets atrial fibrillation:a study in awake chronically instrumented goats[J].Circulation,1995,92:1954-1968.
[11]	MITCHELL P S,PARKIN R K,KROH E M,et al.Circulating microRNAs as stable blood-based markers for cancer detection[J].Proc Natl Acad Sci USA,2008,105:10513-10518.
[12]	MCMANUS D D,LIN H,TANRIVERDI K,et al.Relations between circulating microRNAs and atrial fibrillation:data from the Framingham Offspring Study[J].Heart Rhythm,2014,11:663-669.
[13]	ZHANG Q,KANDIC I,KUTRYK M J.Dysregulation of angiogenesis-related micrornas in endothelial progenitor cells from patients with coronary artery disease[J].Biochem Biophys Res Commun,2011,405:42-46.
[14]	RONKAINEN J J,HANNINEN S L,KORHONEN T,et al.Ca²⁺ -calmodulin-dependent protein kinaseⅡrepresses cardiac transcription of the l-type calcium channel alpha(1c)-subunit gene(cacna1c)by dream translocation[J].J Physiol,2011;589:2669-2686.
[15]	HIGASHIKUNI Y,SAINZ J,NAKAMURA K,et al.The atp-binding cassette transporter abcg2 protects against pressure overload-induced cardiac hypertrophy and heart failure by promoting angiogenesis and antioxidant response[J].Arterioscler Thromb Vasc Biol,2012;32:654-661.
[16]	NOVIK K L,SPINELLI J J,MACARTHUR A C,et al.Genetic variation in hoafx contributes to risk of non-hodgkin lymphoma[J].Cancer Epidemiol Biomarkers Prev,2007,16:1098-1106.
[17]	LU Y,HOU S,HUANG D,et al.Expression profile analysis of circulating microRNAs and their effects on ion channels in Chinese atrial fibrillation patients[J].Int J Clin Exp Med,2015,8:845-853.
[18]	王玺,邱春光,黄振文,等.血浆miRNA-328在心房颤动中的表达水平及其临床意义[J].中华心血管病杂志,2013,41(2),198-199.