The effect of guanfu base A on the late sodium current and excitation conduction velocity in rabbit ventricular muscle
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摘要: 目的:应用微电极阵(MEA)技术研究关附甲素(GFA)对兔心室肌晚钠电流(INaL)及兴奋传导速度(ECV)的影响。方法:新西兰大白兔64只,随机分成4组:对照组(8只),GFA组(1、3、10 μmol/L,每种浓度各8只),海葵毒素组(ATXⅡ,8只),ATXⅡ+ GFA组(1、3、10 μmol/L,每种浓度各8只)。开胸取出心脏后进行langendroff灌流,用柔性电极贴附左心室,记录各项电生理信号的变化。结果:与对照组相比,GFA 1 μmol/L组、3 μmol/L组、10 μmol/L组心室肌场电位时限(fAPD)分别缩短至(338.88±10.93)、(327.13 ±11.57)、(314.25±9.38)ms(P<0.05),ECV分别减慢至(0.51±0.10)、(0.38±0.11)、(0.25±0.07)m/s(P<0.05);ATXⅡ组fAPD明显延长(P<0.05),ECV则无明显变化(P＞0.05)。与ATXⅡ组相比,ATXⅡ+ GFA 1 μmol/L组、3 μmol/L组、10 μmol/L组心室肌fAPD分别缩短至(349.38±11.67)、 (330.88 ±11.09)、(318.50 ±11.05)ms(P<0.05),ECV分别减慢至(0.51±0.10)、(0.41±0.08)、(0.30±0.07)m/s(P<0.05),其fAPD缩短率明显高于GFA组。结论:GFA具有抑制INaL与减缓ECV的作用,可能为其抗心律失常的主要机制。Abstract: Objective: To explore the effect of guanfu base A(GFA)on the late sodium current (INaL) and excitation conduction velocity (ECV) in rabbit ventricular muscle by Microelectrode array(MEA)technology. Method: Sixty four rabbits of either sex were randomly divided into 4 groups:control group(n=8),GFA group (1, 3, and 10 μmol/L, n=8 for each concentration ),ATXⅡ group (n=8), ATXⅡ+ GFA group (1, 3, and 10 μmol/L, n=8 for each concentration ). The heart was perfused with modified Tyrode's solution after removed. Flexible electrodes were attached to the left ventricle, and recorded the changes of electrophysiological signals. Result: In the GFA 1, 3, and 10 μmol/L groups , fAPD shortened to 338.88±10.93, 327.13±11.57, and 314.25±9.38 ms, respectively (P<0.05), while ECV decelerated to 0.51±0.10, 0.38±0.11, and 0.25±0.07 m/s, respectively (P<0.05). In the ATXⅡ group, fAPD prolonged noticeably (P<0.05), however, there was no change in CV (P<0.05). In the ATXⅡ+ GFA 1, 3, and 10 μmol/L groups, fAPD shortened to 349.38±11.67, 330.88 ±11.09, 318.50±11.05 ms, respectively(P<0.05), while ECV decelerated to 0.51±0.10, 0.41±0.08, 0.30±0.07 m/s, respectively (P<0.05), the shortening rate in the ATX+GFA group was significantly higher than that in the GFA group (P<0.05). Conclusion: GFA can inhibit INaL and decelerate ECV in rabbit ventricular muscle, and it may be the primary mechanism of its antiarrhythmic.
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