HMGB1及HMGB2与冠状动脉钙化相关性的分析

刘林鑫; 孟华; 杨冠旭; 李蒙; 刘军; 陈东昌; 李文博; 李震南; 滑世轩; 安松涛; 王忠民; 陈岩

doi:10.13201/j.issn.1001-1439.2021.12.004

HMGB1及HMGB2与冠状动脉钙化相关性的分析

- 1. 河南大学人民医院河南省人民医院心内科(郑州, 450003);
- 2. 河南省人民医院心脏中心华中阜外医院心内科;
- 3. 河南省人民医院心脏中心华中阜外医院检验科
基金项目:
河南省医学科技攻关省部共建项目(No:SB201901095)

详细信息

通讯作者: 陈岩,E-mail:doctorchen04@163.com

中图分类号: R541.4

收稿日期: 2021-07-14

The relationship between HMGB1, HMGB2 and coronary artery calcification

- 1. Department of Cardiology, He'nan Provincial People's Hospital, People's Hospital of He'nan University;
- 2. Department of Cardiology, Central China Fuwai Hospital, He'nan Provincial People's Hospital, Zhengzhou, 450003, China;
- 3. Department of Clinical Laboratory, Central China Fuwai Hospital, He'nan Provincial People's Hospital

More Information

Corresponding author: CHEN Yan, E-mail: doctorchen04@163.com

Received Date: 14 July 2021

摘要

摘要: 目的:探讨血清高迁移率族蛋白1(HMGB1)、血清高迁移率族蛋白2(HMGB2)水平与冠状动脉钙化(CAC)的关系。方法:入选2020年1月—2021年1月于华中阜外医院住院的156例冠心病患者作为试验对象,记录所有患者病史资料、HMGB1以及HMGB2等指标,并进行CAC的分析。将冠心病患者分为非CAC组(62例)与CAC组(94例),比较两组患者临床指标的差异。采用ROC曲线分析HMGB1与HMGB2对CAC的诊断价值,采用logistic回归分析探讨CAC的危险因素。结果:与非CAC组比较,CAC组患者年龄、冠心病病程、BMI、尿酸(UA)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(HbA1c)、高血压病、糖尿病、HMGB1和HMGB2水平均显著升高(均P<0.05),左室射血分数(LVEF)与总胆红素(TBil)水平显著降低(均P<0.05)。ROC曲线显示,HMGB1水平诊断CAC的最佳截点值为6.1773 ng/mL,曲线下面积为0.734(95%CI:0.655~0.813),敏感性为86.2%,特异性为50.0%;HMGB2水平诊断CAC的最佳截点值为5.0396 ng/mL,曲线下面积为0.814(95%CI:0.748~0.881),敏感性为70.2%,特异性为87.1%。多因素logistic回归分析结果显示,TBil是CAC的保护因素,年龄、UA、TG、HbA1c、高血压病、HMGB1、HMGB2是CAC的危险因素。结论:高HMGB1、HMGB2是发生CAC的独立危险因素。
- 血清高迁移率族蛋白1 /
- 血清高迁移率族蛋白2 /
- 钙化 /
- 冠心病
Abstract: Objective: To investigate the relationship between high mobility group box 1(HMGB1), high mobility group box 2(HMGB2), and coronary artery calcification(CAC).Methods: A total of 156 patients with coronary heart disease admitted to Huazhong Fuwai Hospital from January 2020 to January 2021 were enrolled in the study. All patients' medical history, HMGB1, and HMGB2 were recorded, CAC was analyzed. Patients with coronary heart disease were divided into the non-CAC Group(n=62) and CAC group(n=94). The differences in clinical indexes between the two groups were compared. ROC curve was used to analyze the diagnostic value of HMGB1 and HMGB2 in CAC, and logistic regression analysis was used to explore the risk factors of CAC.Results: Compared with the non-CAC group, age, course of coronary heart disease, BMI, uric acid(UA), total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL-C), glycosylated hemoglobin(HbA1 c), hypertension, diabetes, HMGB1, and HMGB2 levels in CAC group were significantly increased(all P<0.05), left ventricular ejection fraction(LVEF) and total bilirubin(TBil) level were significantly decreased(all P<0.05). The ROC analysis showed that the best cut-off point of HMGB1 level in diagnosing CAC was 6.1773 ng/mL, the area under the curve was 0.734(95%CI: 0.655 to 0.813), the sensitivity was 86.2%, and the specificity was 50.0%; The best cut-off value of HMGB2 level in diagnosing CAC was 5.0396 ng/mL, the area under the curve was 0.814(95%CI: 0.748 to 0.881), the sensitivity was 70.2%, and the specificity was 87.1%. Multivariate logistic regression analysis showed that TBil was the protective factor of CAC, and age, UA, TG, HbA1 c, hypertension, HMGB1, and HMGB2 were the risk factors of CAC.Conclusion: High HMGB1 and HMGB2 are independent risk factors for CAC.
- serum high mobility group protein 1 /
- serum high mobility group protein 2 /
- calcification /
- coronary heart disease

HTML全文

参考文献(24)

[1]	Dalen JE,Alpert JS,Goldberg RJ,et al.The epidemic of the 20(th)century:coronary heart disease[J].Am J Med,2014,127(9):807-812.
[2]	Kay FU,Canan A,Abbara S.Future directions in coronary CT angiography:CT-fractional flow reserve,plaque vulnerability,and quantitative plaque assessment[J].Korean Circ J,2020,50(3):185-202.
[3]	Guo J,Erqou SA,Miller RG,et al.The role of coronary artery calcification testing in incident coronary artery disease risk prediction in type 1 diabetes[J].Diabetologia,2019,62(2):259-268.
[4]	Thomas JO,Stott K.H1 and HMGB1:modulators of chromatin structure[J].Biochem Soc Trans,2012,40(2):341-346.
[5]	Yang J,Shah R,Robling AG,et al.HMGB1 is a bone-active cytokine[J].J Cell Physiol,2008,214(3):730-739.
[6]	Barreiro Alonso A,Lamas Maceiras M,Rodriguez Belmonte E,et al.High mobility group B proteins,their partners,and other redox sensors in ovarian and prostate cancer[J].Oxid Med Cell Longev,2016:5845061.
[7]	He YH,Wang XQ,Zhang J,et al.Association of serum HMGB2 levels with in-stent restenosis:HMGB2 promotes neointimal hyperplasia in mice with femoral artery injury and proliferation and migration of VSMCs[J].Arterioscler Thromb Vasc Biol,2017,37(4):717-729.
[8]	Scanlon PJ,Faxon DF,Audet AM,et al.ACC/AHA guidelines for coronary angiography:executive summary and recommendations.A report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Committee on Coronary Angiography) developed in collaboration with the Society for Cardiac Angiography and Interventions[J].Circulation,1999,99(17):2345-2357.
[9]	徐晓艳,唐啸.HMGB1通过糖基化终末产物受体在动脉粥样硬化发病中的作用[J].临床心血管病杂志,2018,34(9):861-865.
[10]	Kalinina N,Agrotis A,Antropova Y,et al.Increased expression of the DNA-binding cytokine HMGB1 in human atherosclerotic lesions:role of activated macrophages and cytokines[J].Arterioscler Thromb Vasc Biol,2004,24(12):2320-2325.
[11]	Lazaros G,Antonopoulos AS,Tousoulis D.The impact of interleukin-18 and high-mobility group box 1 protein signaling in aortic valve calcification[J].Cardiology,2016,135(3):165-167.
[12]	Bostrom KI.Where do we stand on vascular calcification?[J].Vasc Pharmacol,2016,84:8-14.
[13]	Ye Y,Bian W,Fu F,et al.Selenoprotein S inhibits inflammation-induced vascular smooth muscle cell calcification[J].J Biol Inorg Chem,2018,23(5):739-751.
[14]	Longenecker CT,Jiang Y,Orringer CE,et al.Soluble CD14 is independently associated with coronary calcification and extent of subclinical vascular disease in treated HIV infection[J].AIDS,2014,28(7):969-977.
[15]	Lazaros G,Antonopoulos AS,Tousoulis D.The impact of interleukin-18 and high-mobility group Box 1 protein signaling in aortic valve calcification[J].Cardiology,2016,135(3):165-167.
[16]	Taguchi A,Blood DC,del Toro G,et al.Blockade of RAGE-amphoterin signalling suppresses tumour growth and metastases[J].Nature,2000,405(6784):354-360.
[17]	Andersson U,Yang H,Harris H.High-mobility group box 1 protein(HMGB1) operates as an alarmin outside as well as inside cells[J].Semin Immunol,2018,38:40-48.
[18]	Chen Q,Bei JJ,Liu C,et al.HMGB1 induces secretion of matrix vesicles by macrophages to enhance ectopic mineralization[J].PLoS One,2016,11(5):e0156686.
[19]	Wang Y,Shan J,Yang W,et al.High mobility group box 1(HMGB1) mediates high-glucose-induced calcification in vascular smooth muscle cells of saphenous veins[J].Inflammation,2013,36(6):1592-604.
[20]	Barreiro-Alonso A,Lamas-Maceiras M,Rodriguez-Belmonte E,et al.High mobility group B proteins,their partners,and other redox sensors in ovarian and prostate cancer[J].Oxid Med Cell Longev,2016:5845061.
[21]	Hrycek E,Franek A,Baszczak E,et al.Serum levels of selected chemokines in systemic lupus erythematosus patients[J].Rheumatol Int,2013,33(9):2423-2427.
[22]	Liu ZH,Dai DP,Ding FH,et al.Association of serum HMGB2 level with MACE at 1 mo of myocardial infarction:Aggravation of myocardial ischemic injury in rats by HMGB2 via ROS[J].Am J Physiol Heart Circ Physiol,2017,312(3):H422-H436.
[23]	陆志峰,蒋建宇,冯晓波.血清高迁移率族蛋白B2与经皮冠状动脉介入术后再狭窄的关系分析[J].中国循证心血管医学杂志,2019,11(1):38-40,47.
[24]	Ye Y,Bian W,Fu F,et al.Selenoprotein S inhibits inflammation-induced vascular smooth muscle cell calcification[J].J Biol Inorg Chem,2018,23(5):739-751.