Correlation between long non-coding RNA taurine up-regulated gene 1 and coronary in-stent restenosis
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摘要: 目的 探讨长链非编码RNA(LncRNA)牛磺酸上调基因1(TUG1)在冠状动脉(冠脉)支架内再狭窄(ISR)患者外周血中的表达水平，研究TUG1对支架内再狭窄的诊断价值。方法 选取2019年5月—2021年6月于济宁医学院附属医院心内科住院且既往置入过冠脉支架的患者，入院复查冠脉造影评估冠脉支架是否狭窄，根据冠脉造影结果分为支架内再狭窄(ISR)组(26例)和支架内非狭窄(N-ISR)组(26例)。首先收集患者外周血，用单核细胞分离液提取单核细胞，从上述细胞中提取RNA，然后采用实时荧光定量聚合酶链式反应(qRT-PCR)法检测LncRNA TUG1表达水平；采用多因素logistic回归分析研究ISR患者的独立危险因素，采用受试者工作特征曲线(ROC曲线)来评估TUG1对ISR的诊断价值。结果 ISR组TUG1表达量高于N-ISR组[(0.1982±0.2276)∶(0.0704±0.0869)，P< 0.05]，差异有统计学意义。多因素logistic回归分析表明，TUG1为ISR的独立危险因素(OR=1.934，95%CI1.017~3.677，P=0.044)。TUG1的ROC曲线下面积为0.703，灵敏度为65.4%，特异度为73.1%。结论 ISR组患者TUG1的表达水平增高。高表达的TUG1是ISR的独立危险因素，TUG1可能是预测经皮冠脉介入治疗术后发生ISR的新型生物标志物。
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关键词:
- 冠心病 /
- 冠脉支架内再狭窄 /
- 长链非编码RNA牛磺酸上调基因1 /
- 经皮冠状动脉介入治疗 /
- 生物标志物
Abstract: Objective To investigate the expression level of long non-coding RNA(lncRNA) taurine up-regulated gene 1(TUG1) in peripheral blood of patients with coronary in-stent restenosis(ISR), and to study the diagnostic value of TUG1 for in-stent restenosis.Methods Patients who were hospitalized in the Department of Cardiology, Affiliated Hospital of Jining Medical University from May 2019 to June 2021 and had previously received coronary stent implantation, were selected. At the same time, coronary angiography was reexamined at this admission to assess whether the coronary stent was stenosed. The patients were divided into in-stent restenosis(ISR) group(n=26) and in-stent non-stenosis(N-ISR) group(n=26) according to the results of coronary angiography. First, peripheral blood was collected from patients, monocytes were extracted with monocyte isolation solution, RNA was extracted from the above cells, and then lncRNATUG1 expression levels were detected by real-time polymerase chain reaction(qRT-PCR). Multivariate logistic regression analysis was used to study the independent risk factors of ISR patients, and receiver operating characteristic curve(ROC curve) was used to assess the diagnostic value of TUG1 for ISR.Results The expression of TUG1 in the ISR group was higher than that in the N-ISR group[(0.1982±0.2276): (0.0704±0.0869),P< 0.05], and the difference was statistically significant. Multivariate logistic regression analysis showed that TUG1 was an independent risk factor for ISR(OR=1.934, 95%CI1.017~3.677,P=0.044). The area under the ROC curve(AUC) of TUG1 was 0.703, the sensitivity was 65.4%, and the specificity was 73.1%.Conclusion The expression level of TUG1 was increased in ISR patients. High expression of TUG1 was an independent risk factor for ISR, and TUG1 may be a novel biomarker for predicting ISR after percutaneous coronary intervention. -
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表 1 基线资料比较
Table 1. General data
例(%), X±S 基线变量 ISR组(26例) N-ISR组(26例) t/χ2 P值 男性/例(%) 19(73.1) 13(50.0) 2.925 0.087 年龄/岁 63.31±11.40 63.96±10.39 -0.216 0.830 收缩压/mmHg 133.96±20.37 134.35±18.59 -0.071 0.940 舒张压/mmHg 76.38±9.782 79.96±10.91 -1.245 0.220 BMI/(kg·m-2) 26.17±3.25 26.24±3.38 -0.76 0.940 吸烟史/例(%) 17(65.4) 7(26.9) 7.738 0.005 糖尿病/例(%) 15(57.7) 6(23.1) 6.47 0.011 饮酒史/例(%) 10(38.5) 9(34.6) 0.083 0.773 高血压病/例(%) 18(69.2) 17(65.4) 0.087 0.768 TG/(mmol·L-1) 1.32±0.49 1.39±0.64 -0.39 0.700 TC/(mmol·L-1) 3.56±0.98 3.90±0.98 -1.227 0.230 LDL-C/(mmol·L-1) 2.12±0.80 2.25±0.79 -0.579 0.570 HDL-C/(mmol·L-1) 1.13±0.28 1.35±0.36 -2.505 0.020 脂蛋白a/(mg·L-1) 348.73±373.68 451.12±404.59 -0.948 0.350 尿素/(mmol·L-1) 5.13±1.75 5.29±1.67 -0.34 0.740 肌酐/(μmol·L-1) 67.26±12.31 69.30±15.61 -0.524 0.600 ALT/(U·L-1) 22.86±12.79 27.87±28.02 -0.829 0.410 AST/(U·L-1) 22.73±14.17 23.46±11.95 -0.2 0.840 WBC/(×109·L-1) 7.04±1.59 7.47±2.42 -0.757 0.450 HB/(g·L-1) 135.96±16.41 136.69±16.83 -0.159 0.880 PLT/(×109·L-1) 244.81±69.69 247.69±54.77 -0.166 0.870 D-二聚体/(mg·L-1) 0.32±0.15 0.51±0.28 -2.998 0.005 肌酸激酶/(U·L-1) 113.38±95.52 115.81±61.41 -0.109 0.914 LVEF/% 57.81±6.35 59.19±2.50 -1.035 0.308 CK-MB/(ng·mL-1) 5.67±9.03 5.66±13.21 0.003 0.997 乳酸脱氢酶/(U·L-1) 221.31±185.98 187.85±22.40 0.911 0.371 a-羟丁酸脱氢酶/(U·L-1) 179.19±170.00 151.00±19.00 0.84 0.408 
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表 2 多因素Logistic回归分析结果
Table 2. Logistic analysis
变量 β Wald χ2 OR 95%CI P值 吸烟 1.435 4.296 4.200 1.081~16.316 0.038 糖尿病 1.835 6.509 6.267 1.530~25.666 0.011 TUG1 6.593 4.044 1.934 1.017~3.677 0.044
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