Effects of nardostachyos on cardiac sympathetic nerve remodeling after myocardial infarction in rats
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摘要: 目的:探讨甘松对大鼠心肌梗死后交感神经重构的影响。方法:将SD大鼠随机分为假手术组、模型组和甘松组,每组15只。采用结扎大鼠左冠状动脉前降支制备心肌梗死模型。免疫组织化学法检测各组大鼠梗死周边区生长相关蛋白43(GAP43)和酪氨酸羟化酶(TH)阳性神经纤维密度。采用程序刺激各实验组大鼠,诱发室性心律失常。检测心肌组织中超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果:与假手术组比较,模型组阳性神经纤维密度明显增加,室性心律失常诱发率明显升高(均P<0.05);与模型组比较,甘松组大鼠阳性神经纤维密度降低,室性心律失常诱发率减少(均P<0.05)。与假手术组相比,模型组大鼠MDA水平增高,SOD活力降低(均P<0.05);与模型组比较,甘松组大鼠MDA水平降低,SOD活力升高(均P<0.01)。结论:甘松可以有效改善心肌梗死大鼠交感神经重构,预防室性心律失常,其机制可能与改善氧化应激有关。Abstract: Objective:To investigate the effects and the mechanism of nardostachyos on cardiac sympathetic nerve remodeling after myocardial infarction in rats.Method:SD rats were randomly divided into sham group,model group and nardostachyos group(15in each group).Left anterior descending coronary artery was ligated to creat myocardial infarction model.Immunohistochemical technique was used to detect the distribution and density of growth associated protein-43(GAP-43)and tyrosine hydroxylase(TH)positive staining nerve fibers at peri-infarct zone after myocardial infarction.The procedure was used to stimulate rats to induce ventricular arrhythmias.The content of heart tissue malondialdehyde(MDA)and the activity of the superoxide dismutase(SOD)were detected.Result:Compared with sham group,the densities of both GAP-43 and TH positive staining nerve fibers and the incidence of ventricular arrhythmias were significantly higher in model group.Nardostachyos markedly reduced the incidence of ventricular arrhythmias and the densities of both GAP-43 and TH positive staining nerve fibers(all P<0.05).Compared with sham group,the activity of MDA the modle group increased,the activity of SOD decreased significantly,nardostachyos markedly reduced the activity of MDA and increased the activity of SOD of model group(all P<0.05).Conclusion:The amelioration of oxidative stress by nardostachyos may attribute to the improvement in cardiac sympathetic nerve remodeling and the reduction of the ventricular arrhythmia.
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Key words:
- myocardial infarction /
- nardostachyos /
- sympathetic nerve remodeling
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