病毒性心肌炎患者外周血中GARP<sup>+</sup>调节性T细胞的变化

熊晓昉; 蒋雯; 钟禹成; 魏玉珍; 曾秋棠

doi:10.13201/j.issn.1001-1439.2017.09.011

病毒性心肌炎患者外周血中GARP⁺调节性T细胞的变化

- 1 武汉市第三医院心内科(武汉, 430060);
- 2 华中科技大学同济医学院附属协和医院心内科

详细信息

通讯作者: 曾秋棠, E-mail:zengqiutang007@163.com

中图分类号: R542.2

收稿日期: 2017-04-10

Changes of CD4⁺CD25⁺GARP⁺ regulatory T cells in peripheral blood of patients with viral myocarditis

- 1 Department of Cardiology, the Third Hospital of Wuhan, Wuhan, 430060, China;
- 2 Department of Cardiology, Union Hospital, TongJi Medical College, Huazhong University of Science and Technology

More Information

Corresponding author: ZENG Qiutang, E-mail: zengqiutang007@163.com

Received Date: 10 April 2017

摘要

摘要: 目的:观察病毒性心肌炎(viral myocarditis,VMC)患者外周血中CD4⁺CD (25)⁺GARP⁺调节性T细胞的数量和功能变化,以探讨VMC发病的免疫病理机制。方法:选取急性VMC患者25例(VMC组)及与其年龄匹配的健康志愿献血者25例(对照组),采用流式细胞术检测VMC患者外周血中CD4⁺CD (25)⁺GARP⁺调节性T细胞的百分率;实时荧光定量RT-PCR检测FOXP3、GARP以及相关炎性因子的变化;酶联免疫吸附试验(ELISA)检测血清中IFN-γ和IL-17的水平。结果:VMC组与对照组相比,外周血中CD4⁺CD (25)⁺调节性T细胞的比例并未出现明显变化,而CD4⁺CD (25)⁺GARP⁺调节性T细胞的百分率明显下降,GARP的mRNA表达量也较对照组下降,而IL-17和IFN-γ的mRNA表达却显著升高,同时VMC组患者血清中的IFN-γ和IL-17的表达水平却明显高于对照组。结论:CD4⁺CD (25)⁺GARP⁺调节性T细胞在急性VMC患者外周血中的数量和调节功能受到损伤。
- 病毒性心肌炎 /
- GARP+调节性T细胞 /
- 免疫调节
Abstract: Objective:To observe the quantitative and functional changes of CD4⁺CD25⁺GARP⁺ regulatory T cells in peripheral blood of patients with viral myocarditis (VMC).Method:Twenty-five VCM patients and 25 controls were enrolled in this study.Percentages of CD4⁺CD25⁺GARP⁺ regulatory T cells were determined by flowcytometry.The expression levels of FOXP3、GARP and relative inflammatory factor were detected by RT-PCR, and the concentrations of IL-17、IFN-γwere determined by ELISA.Result:The percentage of CD4⁺CD25~regulatory T cells showed no significant difference between VMC patients and control groups, but the percentage of CD4⁺CD25⁺GARP⁺ regulatory T cells was lower in VMC patients.The mRNA expression of GARP was also lower in VMC patients compared to the control group, however, the mRNA expression of IL-17 and IFN-γwere obviously higher in VMC patients.Additionally, the expression levels of IL-17 and IFN-γwere increased in the plasma of VMC patients. Conclusion:The number and function of CD4⁺CD25⁺GARP⁺ regulatory T cells are compromised in VMC patients.
- viral myocarditis /
- GARP+regulatory T cells /
- immune

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