年轻原发性高血压患者T淋巴细胞活化状态及炎性因子表达的研究

操明; 阮磊; 吴晓芬; 黄毅; 张乐; 张存泰; 杨艺

doi:10.13201/j.issn.1001-1439.2021.11.015

年轻原发性高血压患者T淋巴细胞活化状态及炎性因子表达的研究

- 华中科技大学同济医学院附属同济医院综合科 (武汉, 430030)
基金项目:
湖北省自然科学基金(No:2020CFB476)

湖北省自然科学基金(No:2020CFB316)

详细信息

通讯作者: 杨艺,E-mail:yangyi@tjh.tjmu.edu.cn

中图分类号: R544.1

收稿日期: 2021-08-22

The activation of T lymphocytes and expression of inflammatory indicators in young patients with essential hypertension

- Department of Gerontology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

More Information

Corresponding author: YANG Yi, E-mail: yangyi@tjh.tjmu.edu.cn

Received Date: 22 August 2021

摘要

摘要: 目的:探讨年轻原发性高血压患者外周血T淋巴细胞活化状态及炎性因子表达水平。方法:收集36例年龄小于60岁的高血压患者及36例同年龄段的健康成年人的外周血,对所有研究对象均分离外周血单个核细胞,通过流式细胞术检测CD45RA⁺T淋巴细胞和CD45RO⁺T淋巴细胞的比例,ELISA法检测炎性因子白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α的表达,分析CD45RA和CD45RO表达的相对关系,及炎性因子IL-6、TNF-α在年轻人群原发性高血压发病机制中的作用。结果:与健康对照组相比较,年轻原发性高血压患者外周血CD45RA⁺T淋巴细胞的比例显著降低[(42.85±10.62)%∶(33.72±12.40)%,P<0.01],而CD45RO⁺T淋巴细胞的比例显著升高[(57.16±10.62)%∶(66.28±12.40)%,P<0.01];炎性因子IL-6和TNF-α表达水平明显升高[(2.96±0.92) pg/mL∶(3.90±1.26) pg/mL,P<0.01;(2.25±0.39) pg/mL∶(2.56±0.49) pg/mL,P<0.01]。结论:外周血T淋巴细胞过度活化及炎性因子高水平表达,可能参与了年轻人群原发性高血压的发病。
- 原发性高血压 /
- T淋巴细胞 /
- 炎症
Abstract: Objective: To investigate the activation status of T lymphocytes and the expression of inflammatory indicators in peripheral blood of young patients with essential hypertension.Methods: Peripheral bloods of 36 hypertensive patients younger than 60 years old and 36 age-pared healthy adults were collected, and the mono-nuclear cells were separated from the peripheral of all subjects. The frequency of CD45 RA⁺T lymphocytes and CD45 RO⁺T lymphocytes were detected by flow cytometry. ELISA was used to detect the expression of inflammatory indicators IL-6 and TNF-α; and to analyze the relative relationship between CD45 RA and CD45 RO expression and the role of inflammatory indicators IL-6 and TNF-α in the pathogenesis of essential hypertension in young population.Results: Compared with healthy controls, the frequency of CD45 RA⁺T lymphocytes in peripheral blood of young patients with essential hypertension was significantly decreased[(42.85±10.62)% vs(33.72±12.40)%, P<0.01], while the frequency of CD45 RO⁺T lymphocytes was significantly increased[(57.16±10.62)% vs(66.28±12.40)%, P<0.01]. The expression levels of inflammatory indicators IL-6 and TNF-α were significantly increased[(2.96±0.92) pg/mL vs(3.90±1.26) pg/mL, P<0.01;(2.25±0.39) pg/mL vs(2.56±0.49) pg/mL, P<0.01].Conclusion: Excessive activation of T lymphocytes and high expression of inflammatory indicators in the peripheral blood may be involved in the pathogenesis of essential hypertension in young people.
- essential hypertension /
- T lymphocytes /
- inflammation

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